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验证数据展示

  • WB analysis using 82371-5-RR (same clone as 82371-5-PBS)

    WB analysis using 82371-5-RR (same clone as 82371-5-PBS)

    HEK-293 cells and NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82371-5-RR (Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) antibody) blocked with BSA only, Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) peptide or non-Phospho peptide at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control. This data was developed using the same antibody clone with 82371-5-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 82371-5-RR (same clone as 82371-5-PBS)

    FC experiment of HEK-293 using 82371-5-RR (same clone as 82371-5-PBS)

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.25 ug Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) Recombinant monoclonal antibody (82371-5-RR, Clone:242663E11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 90% MeOH . This data was developed using the same antibody clone with 82371-5-PBS in a different storage buffer formulation.

Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) Recombinant monoclonal antibody, PBS Only

Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 82371-5-PBS

产品说明书

CloneNo. 242663E11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Indirect ELISA

别名

P-GSK3A (Tyr279), P-GSK3B (Tyr216), Phos-GSK3 beta (Tyr216), Phos-GSK3A (Tyr279), Phos-GSK3B (Tyr216)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GSK3B Ready-To-Use IHC Kit. GSK3B primary antibody included.

产品信息

82371-5-PBS targets Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glycogen synthase kinase 3 beta
别名 P-GSK3A (Tyr279), P-GSK3B (Tyr216), Phos-GSK3 beta (Tyr216), Phos-GSK3A (Tyr279), Phos-GSK3B (Tyr216)
计算分子量 433 aa, 48 kDa
观测分子量48-50 kDa
GenBank蛋白编号BC000251
基因名称 GSK3B
Gene ID (NCBI) 2932
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP49841
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glycogen synthase kinase 3 (GSK3) proteins are serine/threonine kinases that were originally identified as key regulatory enzymes in glucose metabolism. There are two isoforms, GSK3 alpha and GSK3 beta, that are encoded by separate genes and that are 85% homologous to each other overall with 95% homology in the kinase domains. GSK3 beta is activated by phosphorylation of the tyrosine 216 residue (Tyr216) located in the kinase domain and inactivated by phosphorylation of the amino-terminal serine 9 residue (Ser9). GSK3 phosphorylation inhibits its catalytic activity towards its substrates, allowing cytosolic stabilization and nuclear translocation of the transcriptional targets, β-catenin and Nrf2. (PMID: 31354196 , PMID: 25750704,PMID: 24722055)

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