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验证数据展示

  • WB analysis using 82371-5-RR

    WB analysis using 82371-5-RR

    HEK-293 cells and NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82371-5-RR (Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) antibody) blocked with BSA only, Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) peptide or non-Phospho peptide at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control.

  • FC experiment of HEK-293 using 82371-5-RR

    FC experiment of HEK-293 using 82371-5-RR

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.25 ug Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) Recombinant monoclonal antibody (82371-5-RR, Clone:242663E11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 90% MeOH .

Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) Recombinant monoclonal antibody

Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 82371-5-RR

产品说明书

CloneNo. 242663E11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

P-GSK3A (Tyr279), P-GSK3B (Tyr216), Phos-GSK3 beta (Tyr216), Phos-GSK3A (Tyr279), Phos-GSK3B (Tyr216)

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells
Positive FC (Intra) detected inλ phosphatase treated HEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy GSK3B Ready-To-Use IHC Kit. GSK3B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82371-5-RR targets Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 glycogen synthase kinase 3 beta
别名 P-GSK3A (Tyr279), P-GSK3B (Tyr216), Phos-GSK3 beta (Tyr216), Phos-GSK3A (Tyr279), Phos-GSK3B (Tyr216)
计算分子量 433 aa, 48 kDa
观测分子量48-50 kDa
GenBank蛋白编号BC000251
基因名称 GSK3B
Gene ID (NCBI) 2932
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP49841
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glycogen synthase kinase 3 (GSK3) proteins are serine/threonine kinases that were originally identified as key regulatory enzymes in glucose metabolism. There are two isoforms, GSK3 alpha and GSK3 beta, that are encoded by separate genes and that are 85% homologous to each other overall with 95% homology in the kinase domains. GSK3 beta is activated by phosphorylation of the tyrosine 216 residue (Tyr216) located in the kinase domain and inactivated by phosphorylation of the amino-terminal serine 9 residue (Ser9). GSK3 phosphorylation inhibits its catalytic activity towards its substrates, allowing cytosolic stabilization and nuclear translocation of the transcriptional targets, β-catenin and Nrf2. (PMID: 31354196 , PMID: 25750704,PMID: 24722055)

实验方案

Product Specific Protocols
FC protocol for Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) antibody 82371-5-RRDownload protocol
WB protocol for Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) antibody 82371-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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