验证数据展示
经过测试的应用
| Positive WB detected in | EGF treated A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87513-1-RR targets Phospho-Filamin A (Ser2152) in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | filamin A, alpha (actin binding protein 280) |
| 别名 | p-Filamin A (S2152), p-Filamin A Ser2152, p-FLNA Ser2152, Phospho Filamin A (Ser2152), Phospho FLNA (Ser2152) |
| 计算分子量 | 2647 aa, 280 kDa |
| 观测分子量 | 280 kDa |
| GenBank蛋白编号 | NM_001110556 |
| 基因名称 | FLNA |
| Gene ID (NCBI) | 2316 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P21333 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
FLNA encodes a 280 kDa widely expressed actin binding protein called filamin A which can crosslinks actin filaments and links actin filaments to membrane glycoproteins to form a three-dimensional network. Phospho-Filamin A (Ser2152) is a phosphorylated form of Filamin A (FLNA), which is a large actin-binding protein that plays a crucial role in organizing the cytoskeleton and transducing signals from cell membrane to the cytoskeleton. This phosphorylation event is associated with various cellular functions, including cytoskeletal remodeling, cell migration, and focal adhesion formation. (PMID:29931263)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-Filamin A (Ser2152) antibody 87513-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
