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验证数据展示

  • IF Staining of U2OS using CL488-67931

    IF Staining of U2OS using CL488-67931

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 PTPN9 antibody (CL488-67931, Clone: 1D6E4 ) at dilution of 1:200.

  • IF Staining of U2OS using CL488-67931

    IF Staining of U2OS using CL488-67931

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CoraLite® Plus 488 PTPN9 antibody (CL488-67931, Clone: 1D6E4 ) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-67931

    FC experiment of MCF-7 using CL488-67931

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human PTPN9 (CL488-67931, Clone:1D6E4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated PTPN9 Monoclonal antibody

PTPN9 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67931

产品说明书

CloneNo. 1D6E4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, pig

应用

IF/ICC, FC (Intra)

别名

MEG2, PTPaseMEG2, PTPase MEG2, Protein-tyrosine phosphatase MEG2, EC:3.1.3.48

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 67931-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67931 targets PTPN9 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, pig
免疫原

CatNo: Ag30615

Product name: Recombinant human PTPN9 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 248-593 aa of BC010863

Sequence: FQFLPQVNGHPDPFDEIILFSLPPALDWDSVHVPGPHAMTIQELVDYVNARQKQGIYEEYEDIRRENPVGTFHCSMSPGNLEKNRYGDVPCLDQTRVKLTKRSGHTQTDYINASFMDGYKQKNAYIGTQGPLENTYRDFWLMVWEQKVLVIVMTTRFEEGGRRKCGQYWPLEKDSRIRFGFLTVTNLGVENMNHYKKTTLEIHNTEERQKRQVTHFQFLSWPDYGVPSSAASLIDFLRVVRNQQSLAVSNMGARSKGQCPEPPIVVHCSAGIGRTGTFCSLDICLAQLEELGTLNVFQTVSRMRTQRAFSIQTPEQYYFCYKAILEFAEKEGMVSSGQNLLAVESQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, non-receptor type 9
别名 MEG2, PTPaseMEG2, PTPase MEG2, Protein-tyrosine phosphatase MEG2, EC:3.1.3.48
计算分子量 593 aa, 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC010863
基因名称 PTPN9
Gene ID (NCBI) 5780
RRIDAB_2923831
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP43378
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN9, Tyrosine-protein phosphatase non-receptor type 9, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN9 is involved in the transfer of hydrophobic ligands or in functions of the Golgi apparatus (PMID: 19167335). MiR-96 inhibits PTPN9 expression and consequently promotes proliferation, migration and invasion of breast cancer cells (PMID:27857177).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PTPN9 antibody CL488-67931Download protocol
FC protocol for CL Plus 488 PTPN9 antibody CL488-67931Download protocol
Standard Protocols
Click here to view our Standard Protocols