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验证数据展示

  • WB analysis using 67931-1-Ig

    WB analysis using 67931-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67931-1-Ig (PTPN9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 67931-1-Ig

    WB analysis of A549 using 67931-1-Ig

    WB result of PTPN9 antibody (67931-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPN9 transfected A549 cells.

  • IF Staining of U2OS using 67931-1-Ig

    IF Staining of U2OS using 67931-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PTPN9 antibody (67931-1-Ig, Clone: 1D6E4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO验证

PTPN9 Monoclonal antibody

PTPN9 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67931-1-Ig

产品说明书

CloneNo. 1D6E4

宿主/亚型

Mouse / IgG1

种属反应性

Human, pig, mouse

应用

WB, IF/ICC, ELISA

别名

MEG2, PTPase MEG2, PTPMEG2, PTPN9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, Jurkat cells, pig pancreas tissue, A549 cells, Hela cells, RAW 264.7 cells
Positive IF/ICC detected inU2OS cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67931-1-Ig targets PTPN9 in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, pig, mouse
免疫原

CatNo: Ag30615

Product name: Recombinant human PTPN9 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 248-593 aa of BC010863

Sequence: FQFLPQVNGHPDPFDEIILFSLPPALDWDSVHVPGPHAMTIQELVDYVNARQKQGIYEEYEDIRRENPVGTFHCSMSPGNLEKNRYGDVPCLDQTRVKLTKRSGHTQTDYINASFMDGYKQKNAYIGTQGPLENTYRDFWLMVWEQKVLVIVMTTRFEEGGRRKCGQYWPLEKDSRIRFGFLTVTNLGVENMNHYKKTTLEIHNTEERQKRQVTHFQFLSWPDYGVPSSAASLIDFLRVVRNQQSLAVSNMGARSKGQCPEPPIVVHCSAGIGRTGTFCSLDICLAQLEELGTLNVFQTVSRMRTQRAFSIQTPEQYYFCYKAILEFAEKEGMVSSGQNLLAVESQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, non-receptor type 9
别名 MEG2, PTPase MEG2, PTPMEG2, PTPN9
计算分子量 593 aa, 68 kDa
观测分子量 68 kDa
GenBank蛋白编号BC010863
基因名称 PTPN9
Gene ID (NCBI) 5780
RRIDAB_2918683
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP43378
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN9, Tyrosine-protein phosphatase non-receptor type 9, is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN9 is involved in the transfer of hydrophobic ligands or in functions of the Golgi apparatus (PMID: 19167335). MiR-96 inhibits PTPN9 expression and consequently promotes proliferation, migration and invasion of breast cancer cells (PMID:27857177).

实验方案

Product Specific Protocols
WB protocol for PTPN9 antibody 67931-1-IgDownload protocol
IF protocol for PTPN9 antibody 67931-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols