验证数据展示
经过测试的应用
| Positive IF/ICC detected in | Jurkat cells |
For other applications, we recommend the unconjugated version of this antibody, 85062-4-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-85062-4 targets PTPN22 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | PTPN22 fusion protein Ag2397 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | protein tyrosine phosphatase, non-receptor type 22 (lymphoid) |
| 别名 | 242688A2, EC:3.1.3.48, Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, LyP, LYP1 |
| 计算分子量 | 82 kDa, 92 kDa |
| GenBank蛋白编号 | BC017785 |
| 基因名称 | PTPN22 |
| Gene ID (NCBI) | 26191 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9Y2R2 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PTPN22(Tyrosine-protein phosphatase non-receptor type 22) is also named as LyP,PEP,PTPN8.It belongs to the protein-tyrosine phosphatase family and non-receptor class 4 subfamily.It acts as negative regulator of T-cell receptor (TCR) signaling.Defects in PTPN22 are a cause of susceptibility to systemic lupus erythematosus (SLE). It has some isoforms produced by alternative splicing with calculated molecular mass of 79-92 kDa, and apparent molecular mass of 100-110 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 PTPN22 antibody CL488-85062-4 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |