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验证数据展示

  • IF Staining of Jurkat using 85062-4-RR

    IF Staining of Jurkat using 85062-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PTPN22 antibody (85062-4-RR, Clone: 242688A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of Jurkat using 85062-4-RR

    FC experiment of Jurkat using 85062-4-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PTPN22 Recombinant antibody (85062-4-RR, Clone:242688A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

PTPN22 Recombinant monoclonal antibody

PTPN22 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 85062-4-RR

产品说明书

CloneNo. 242688A2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

242688A2, EC:3.1.3.48, Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, LyP, LYP1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85062-4-RR targets PTPN22 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2397

Product name: Recombinant human PTPN22 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-179 aa of BC017785

Sequence: MDQREILQKFLDEAQSKKITKEEFANEFLKLKRQSTKYKADKTYPTTVAEKPKNIKKNRYKDILPYDYSRVELSLITSDEDSSYINANFIKGVYGPKAYIATQGPLSTTLLDFWRMIWEYSVLIIVMACMEYEMGKEAEKRKSDYIIRTLKVKFNSVSVILAHQTSLQNLFSQITPAHF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein tyrosine phosphatase, non-receptor type 22 (lymphoid)
别名 242688A2, EC:3.1.3.48, Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, LyP, LYP1
计算分子量 82 kDa, 92 kDa
GenBank蛋白编号BC017785
基因名称 PTPN22
Gene ID (NCBI) 26191
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y2R2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN22(Tyrosine-protein phosphatase non-receptor type 22) is also named as LyP,PEP,PTPN8.It belongs to the protein-tyrosine phosphatase family and non-receptor class 4 subfamily.It acts as negative regulator of T-cell receptor (TCR) signaling.Defects in PTPN22 are a cause of susceptibility to systemic lupus erythematosus (SLE). It has some isoforms produced by alternative splicing with calculated molecular mass of 79-92 kDa, and apparent molecular mass of 100-110 kDa.

实验方案

Product Specific Protocols
FC protocol for PTPN22 antibody 85062-4-RRDownload protocol
IF protocol for PTPN22 antibody 85062-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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