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CoraLite®555-conjugated PTPN11/SHP2 Monoclonal antibody

PTPN11/SHP2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3F8A8

Cat No : CL555-66795

Print datasheet

Synonyms

BPTP3, CFC, NS1, PTP 1D, PTP 2C, PTP2C, PTPN11, SH PTP2, SH PTP3, SHP 2, SHP2, SHPTP2

验证数据展示

  • IF Staining of MCF-7 using CL555-66795

    IF Staining of MCF-7 using CL555-66795

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CoraLite®555 PTPN11 antibody (CL555-66795, Clone: 3F8A8 ) at dilution of 1:200.

  • FC experiment of MCF-7 using CL555-66795

    FC experiment of MCF-7 using CL555-66795

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human PTPN11 (CL555-66795, Clone:3F8A8) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66795-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66795 targets PTPN11/SHP2 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC (Intra)
Tested Reactivity Human, Mouse, Rat
Immunogen PTPN11/SHP2 fusion protein Ag13649 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, non-receptor type 11
Synonyms BPTP3, CFC, NS1, PTP 1D, PTP 2C, PTP2C, PTPN11, SH PTP2, SH PTP3, SHP 2, SHP2, SHPTP2
Calculated Molecular Weight 597 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC008692
Gene Symbol PTPN11
Gene ID (NCBI) 5781
RRIDAB_2919695
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ06124
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTPN11 (protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism (PMID: 19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions (PMID: 21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL555 PTPN11/SHP2 antibody CL555-66795Download protocol
FC protocol for CL555 PTPN11/SHP2 antibody CL555-66795Download protocol
Standard Protocols
Click here to view our Standard Protocols