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验证数据展示

  • WB analysis using 84928-2-RR (same clone as 84928-2-PBS)

    WB analysis using 84928-2-RR (same clone as 84928-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84928-2-RR (PSMD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84928-2-RR (same clone as 84928-2-PBS)

    IF Staining of HeLa using 84928-2-RR (same clone as 84928-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMD3 antibody (84928-2-RR, Clone: 242470D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 84928-2-RR (same clone as 84928-2-PBS)

    IHC staining of human colon cancer using 84928-2-RR (same clone as 84928-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84928-2-RR (PSMD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMD3 antibody included
  • FC experiment of A549 using 84928-2-RR (same clone as 84928-2-PBS)

    FC experiment of A549 using 84928-2-RR (same clone as 84928-2-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PSMD3 Recombinant antibody (84928-2-RR, Clone:242470D4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84928-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01691-2

    Cytometric bead array standard curve of MP01691-2

    Cytometric bead array standard curve of MP01691-2, PSMD3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84928-2-PBS. Detection antibody: 84928-1-PBS. Standard: Ag2684. Range: 0.781-100 ng/mL.

PSMD3 Recombinant antibody, PBS Only (Capture)

PSMD3 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 84928-2-PBS

产品说明书

CloneNo. 242470D4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

242470D4, 26S proteasome non-ATPase regulatory subunit 3, P58, RPN3, S3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PSMD3 Ready-To-Use IHC Kit. PSMD3 primary antibody included.

产品信息

84928-2-PBS targets PSMD3 as part of a matched antibody pair:

MP01691-2: 84928-2-PBS capture and 84928-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PSMD3 fusion protein Ag2684 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
别名 242470D4, 26S proteasome non-ATPase regulatory subunit 3, P58, RPN3, S3
计算分子量 534 aa, 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC012302
基因名称 PSMD3
Gene ID (NCBI) 5709
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO43242
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSMD3, encoding proteasome (prosome, macropain) 26S subunit, non-ATPase 3, a member of the 26S proteasome family. PSMD3 is expressed in most of the tissues according to the RNA-Seq database. It has been reported that PSMD3 genotypes are associated with white blood cell (WBC) and neutrophil counts and have interactions with dietary fatty acids and carbohydrates on glucose-related traits. PSMD3 is located at ch17q21 and encodes a protein with a molecular weight of 61 kDa (PMID: 31013812).