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验证数据展示

  • WB analysis using 84928-2-RR

    WB analysis using 84928-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84928-2-RR (PSMD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84928-2-RR

    IF Staining of HeLa using 84928-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMD3 antibody (84928-2-RR, Clone: 242470D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of human colon cancer using 84928-2-RR

    IHC staining of human colon cancer using 84928-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84928-2-RR (PSMD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMD3 antibody included
  • FC experiment of A549 using 84928-2-RR

    FC experiment of A549 using 84928-2-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PSMD3 Recombinant antibody (84928-2-RR, Clone:242470D4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

PSMD3 Recombinant antibody

PSMD3 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84928-2-RR

产品说明书

CloneNo. 242470D4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

242470D4, 26S proteasome non-ATPase regulatory subunit 3, P58, RPN3, S3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, U2OS cells, U-251 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, U-251 cells, A549 cells, U2OS cells
Positive FC (Intra) detected inA549 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMD3 Ready-To-Use IHC Kit. PSMD3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84928-2-RR targets PSMD3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PSMD3 fusion protein Ag2684 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
别名 242470D4, 26S proteasome non-ATPase regulatory subunit 3, P58, RPN3, S3
计算分子量 534 aa, 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC012302
基因名称 PSMD3
Gene ID (NCBI) 5709
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO43242
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMD3, encoding proteasome (prosome, macropain) 26S subunit, non-ATPase 3, a member of the 26S proteasome family. PSMD3 is expressed in most of the tissues according to the RNA-Seq database. It has been reported that PSMD3 genotypes are associated with white blood cell (WBC) and neutrophil counts and have interactions with dietary fatty acids and carbohydrates on glucose-related traits. PSMD3 is located at ch17q21 and encodes a protein with a molecular weight of 61 kDa (PMID: 31013812).

实验方案

Product Specific Protocols
WB protocol for PSMD3 antibody 84928-2-RRDownload protocol
IHC protocol for PSMD3 antibody 84928-2-RRDownload protocol
IF protocol for PSMD3 antibody 84928-2-RRDownload protocol
FC protocol for PSMD3 antibody 84928-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols