PSMD3 Recombinant antibody

PSMD3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84928-2-RR

产品说明书

CloneNo. 242470D4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

242470D4, 26S proteasome non-ATPase regulatory subunit 3, P58, RPN3, S3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, U2OS cells, U-251 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, U-251 cells, A549 cells, U2OS cells
Positive FC (Intra) detected inA549 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy PSMD3 Ready-To-Use IHC Kit. PSMD3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84928-2-RR targets PSMD3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PSMD3 fusion protein Ag2684 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
别名 242470D4, 26S proteasome non-ATPase regulatory subunit 3, P58, RPN3, S3
计算分子量 534 aa, 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC012302
基因名称 PSMD3
Gene ID (NCBI) 5709
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43242
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMD3, encoding proteasome (prosome, macropain) 26S subunit, non-ATPase 3, a member of the 26S proteasome family. PSMD3 is expressed in most of the tissues according to the RNA-Seq database. It has been reported that PSMD3 genotypes are associated with white blood cell (WBC) and neutrophil counts and have interactions with dietary fatty acids and carbohydrates on glucose-related traits. PSMD3 is located at ch17q21 and encodes a protein with a molecular weight of 61 kDa (PMID: 31013812).

实验方案

Product Specific Protocols
WB protocol for PSMD3 antibody 84928-2-RRDownload protocol
IHC protocol for PSMD3 antibody 84928-2-RRDownload protocol
IF protocol for PSMD3 antibody 84928-2-RRDownload protocol
FC protocol for PSMD3 antibody 84928-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...