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验证数据展示

  • IHC staining of mouse liver using 84424-4-RR

    IHC staining of mouse liver using 84424-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84424-4-RR (PSMA5 antibody) at dilution of 1:400 (under 40x lens).

    Ready-to-use IHC kits available with PSMA5 antibody included
  • IHC staining of mouse brain using 84424-4-RR

    IHC staining of mouse brain using 84424-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84424-4-RR (PSMA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA5 antibody included
  • IF Staining of HeLa using 84424-4-RR

    IF Staining of HeLa using 84424-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA5 antibody (84424-4-RR, Clone: 241629H2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of U2OS using 84424-4-RR

    IF Staining of U2OS using 84424-4-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSMA5 antibody (84424-4-RR, Clone: 241629H2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of HeLa using 84424-4-RR

    IF Staining of HeLa using 84424-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA5 antibody (84424-4-RR, Clone: 241629H2 ) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • FC experiment of HepG2 using 84424-4-RR

    FC experiment of HepG2 using 84424-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PSMA5 Recombinant antibody (84424-4-RR, Clone:241629H2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 84424-4-RR

    Affinity and Kinetic Characterization of 84424-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84424-4-RR against Human PSMA5 were performed. The affinity constant is 36.6 pM.

PSMA5 Recombinant antibody

PSMA5 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84424-4-RR

产品说明书

CloneNo. 241629H2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

241629H2, Proteasome subunit alpha type-5, PSC5, ZETA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse liver tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, U2OS cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PSMA5 Ready-To-Use IHC Kit. PSMA5 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84424-4-RR targets PSMA5 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 PSMA5 fusion protein Ag34928 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, alpha type, 5
别名 241629H2, Proteasome subunit alpha type-5, PSC5, ZETA
计算分子量 241 aa, 26 kDa
GenBank蛋白编号BC103751
基因名称 PSMA5
Gene ID (NCBI) 5686
RRIDAB_3671951
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP28066
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Proteasome subunit alpha type-5(PSMA5) is a component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. It binds to two 19S regulatory particles(RP) to form the 26S proteasome, an ATP-dependent multisubunit protease that degrades polyubiquitinated proteins into small peptides.(PMID: 26661102)

实验方案

Product Specific Protocols
IHC protocol for PSMA5 antibody 84424-4-RRDownload protocol
IF protocol for PSMA5 antibody 84424-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols