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验证数据展示

  • IF Staining of HeLa using 84424-4-RR (same clone as 84424-4-PBS)

    IF Staining of HeLa using 84424-4-RR (same clone as 84424-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA5 antibody (84424-4-RR, Clone: 241629H2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84424-4-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 84424-4-RR (same clone as 84424-4-PBS)

    IF Staining of U2OS using 84424-4-RR (same clone as 84424-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PSMA5 antibody (84424-4-RR, Clone: 241629H2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84424-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01286-3

    Cytometric bead array standard curve of MP01286-3

    Cytometric bead array standard curve of MP01286-3, PSMA5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84424-4-PBS. Detection antibody: 84424-1-PBS. Standard: Ag34928. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 84424-4-RR

    Affinity and Kinetic Characterization of 84424-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84424-4-RR against Human PSMA5 were performed. The affinity constant is 36.6 pM.

PSMA5 Recombinant antibody, PBS Only (Capture)

PSMA5 Uni-rAbTM Recombinant Antibody for IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84424-4-PBS

产品说明书

CloneNo. 241629H2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, Cytometric bead array, Indirect ELISA

别名

ZETA, PSC5, Proteasome subunit alpha type-5, 241629H2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PSMA5 Ready-To-Use IHC Kit. PSMA5 primary antibody included.

产品信息

84424-4-PBS targets PSMA5 as part of a matched antibody pair:

MP01286-3: 84424-4-PBS capture and 84424-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 PSMA5 fusion protein Ag34928 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 proteasome (prosome, macropain) subunit, alpha type, 5
别名 ZETA, PSC5, Proteasome subunit alpha type-5, 241629H2
计算分子量 241 aa, 26 kDa
GenBank蛋白编号BC103751
基因名称 PSMA5
Gene ID (NCBI) 5686
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP28066
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Proteasome subunit alpha type-5(PSMA5) is a component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. It binds to two 19S regulatory particles(RP) to form the 26S proteasome, an ATP-dependent multisubunit protease that degrades polyubiquitinated proteins into small peptides.(PMID: 26661102)