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验证数据展示

  • WB analysis of human milk using 66473-1-Ig (same clone as 66473-1-PBS)

    WB analysis of human milk using 66473-1-Ig (same clone as 66473-1-PBS)

    human milk were subjected to SDS PAGE followed by western blot with 66473-1-Ig (PSAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66473-1-Ig (same clone as 66473-1-PBS)

    WB analysis of HeLa using 66473-1-Ig (same clone as 66473-1-PBS)

    WB result of PSAP antibody (66473-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HeLa cells. This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

    IHC staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66473-1-Ig (PSAP antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSAP antibody included
  • IHC staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

    IHC staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66473-1-Ig (PSAP antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSAP antibody included
  • IHC staining of mouse kidney using 66473-1-Ig (same clone as 66473-1-PBS)

    IHC staining of mouse kidney using 66473-1-Ig (same clone as 66473-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66473-1-Ig (PSAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSAP antibody included
  • IHC staining of mouse kidney using 66473-1-Ig (same clone as 66473-1-PBS)

    IHC staining of mouse kidney using 66473-1-Ig (same clone as 66473-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66473-1-Ig (PSAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSAP antibody included
  • IHC staining of rat kidney using 66473-1-Ig (same clone as 66473-1-PBS)

    IHC staining of rat kidney using 66473-1-Ig (same clone as 66473-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66473-1-Ig (PSAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSAP antibody included
  • IF Staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

    IF Staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

  • IF Staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

    IF Staining of human prostate cancer using 66473-1-Ig (same clone as 66473-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66473-1-Ig (same clone as 66473-1-PBS)

    IF Staining of HeLa using 66473-1-Ig (same clone as 66473-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSAP antibody (66473-1-Ig, Clone: 2F11B2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66473-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

PSAP Monoclonal antibody, PBS Only

PSAP Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 66473-1-PBS

产品说明书

CloneNo. 2F11B2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

2F11B2, A1 activator, Cerebroside sulfate activator, Co beta glucosidase, Component C

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PSAP Ready-To-Use IHC Kit. PSAP primary antibody included.

产品信息

66473-1-PBS targets PSAP in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1194

Product name: Recombinant human PSAP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-320 aa of BC001503

Sequence: MYALFLLASLLGAALAGPVLGLKECTRGSAVWCQNVKTASDCGAVKHCLQTVWNKPTVKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQKHLAELNHQKQLESNKIPELDMTEVVAPFMANIPLLLYPQDGPRSKPQPKDNGDVCQDCIQMVTDIQTAVRTNSTFVQALVEHVKEECDRLGPGMADICKNYISQYSEIAIQMMMHMQPKEICALVGFCDEVKEMPMQTLVPAKVASKNVIPALELVEPIKKHEVPAKSDVYCEVCEF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 prosaposin
别名 2F11B2, A1 activator, Cerebroside sulfate activator, Co beta glucosidase, Component C
计算分子量 58 kDa
观测分子量 70 kDa
GenBank蛋白编号BC001503
基因名称 PSAP
Gene ID (NCBI) 5660
RRIDAB_2881839
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP07602
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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