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PGRMC1 Monoclonal antibody

PGRMC1 Monoclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C10D11

Cat No : 66372-1-Ig

Print datasheet

Synonyms

HPR6.6, MPR, PGRMC, PGRMC1

验证数据展示

  • WB analysis of HEK-293 using 66372-1-Ig

    WB analysis of HEK-293 using 66372-1-Ig

    WB result of PGRMC1 antibody (66372-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PGRMC1 transfected HEK-293 cells.

  • WB analysis using 66372-1-Ig

    WB analysis using 66372-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66372-1-Ig (PGRMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human ovary tumor using 66372-1-Ig

    IHC staining of human ovary tumor using 66372-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66372-1-Ig (PGRMC1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PGRMC1 antibody included
  • IHC staining of human ovary tumor using 66372-1-Ig

    IHC staining of human ovary tumor using 66372-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66372-1-Ig (PGRMC1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PGRMC1 antibody included
  • IF Staining of human ovary tumor using 66372-1-Ig

    IF Staining of human ovary tumor using 66372-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using PGRMC1 antibody (66372-1-Ig, Clone: 1C10D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human ovary tumor using 66372-1-Ig

    IF Staining of human ovary tumor using 66372-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using PGRMC1 antibody (66372-1-Ig, Clone: 1C10D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, fetal human brain tissue, MCF-7 cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, pig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman ovary tumor tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman ovary tumor tissue
Planning an IHC experiment? We recommend our IHCeasy PGRMC1 Ready-To-Use IHC Kit. PGRMC1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66372-1-Ig targets PGRMC1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF-P, IHC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman
Immunogen PGRMC1 fusion protein Ag3643 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name progesterone receptor membrane component 1
Synonyms HPR6.6, MPR, PGRMC, PGRMC1
Calculated Molecular Weight 195 aa, 22 kDa
Observed Molecular Weight 25-27 kDa
GenBank Accession NumberBC034238
Gene Symbol PGRMC1
Gene ID (NCBI) 10857
RRIDAB_2881751
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00264
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Progesterone receptor membrane component 1 (PGRMC1) is a member of a multi-protein progesterone-binding complex. However, PGRMC1 shares homology with cytochrome b5-related proteins rather than hormone receptors (PMID: 18992768). It is a heme binding protein with biding sites for Src homology (SH2) and SH3 domain-containing proteins (PMID: 17583495). PGRMC1 is overexpressed in a variety of cancers, and thus represents an important biomarker for cancer progression and a potential target for anticancer drugs (PMID: 21730960). In nonmalignant tissues, PGRMC1 is highly expressed in the liver and kidney (PMID: 9705155; 20164297).

实验方案

Product Specific Protocols
WB protocol for PGRMC1 antibody 66372-1-IgDownload protocol
IHC protocol for PGRMC1 antibody 66372-1-IgDownload protocol
IF protocol for PGRMC1 antibody 66372-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF,WB

Biol Chem

A progesterone receptor membrane component 1 antagonist induces large vesicles independent of progesterone receptor membrane component 1 expression.

Authors - Lihua Wang-Eckhardt
View Article