验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, fetal human brain tissue, MCF-7 cells, NIH/3T3 cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, pig brain tissue, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human ovary tumor tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human ovary tumor tissue |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
66372-1-Ig targets PGRMC1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag3643 Product name: Recombinant human PGRMC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 43-195 aa of BC034238 Sequence: YKIVRGDQPAASGDSDDDEPPPLPRLKRRDFTPAELRRFDGVQDPRILMAINGKVFDVTKGRKFYGPEGPYGVFAGRDASRGLATFCLDKEALKDEYDDLSDLTAAQQETLSDWESQFTFKYHHVGKLLKEGEEPTVYSDEEEPKDESARKND 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | progesterone receptor membrane component 1 |
| 别名 | PGRMC, mPR, Membrane-associated progesterone receptor component 1, HPR6.6, 1C10D11 |
| 计算分子量 | 195 aa, 22 kDa |
| 观测分子量 | 25-27 kDa |
| GenBank蛋白编号 | BC034238 |
| 基因名称 | PGRMC1 |
| Gene ID (NCBI) | 10857 |
| RRID | AB_2881751 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O00264 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Progesterone receptor membrane component 1 (PGRMC1) is a member of a multi-protein progesterone-binding complex. However, PGRMC1 shares homology with cytochrome b5-related proteins rather than hormone receptors (PMID: 18992768). It is a heme binding protein with biding sites for Src homology (SH2) and SH3 domain-containing proteins (PMID: 17583495). PGRMC1 is overexpressed in a variety of cancers, and thus represents an important biomarker for cancer progression and a potential target for anticancer drugs (PMID: 21730960). In nonmalignant tissues, PGRMC1 is highly expressed in the liver and kidney (PMID: 9705155; 20164297).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PGRMC1 antibody 66372-1-Ig | Download protocol |
| IHC protocol for PGRMC1 antibody 66372-1-Ig | Download protocol |
| WB protocol for PGRMC1 antibody 66372-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
