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验证数据展示

  • WB analysis of HEK-293 using 66372-1-Ig (same clone as 66372-1-PBS)

    WB analysis of HEK-293 using 66372-1-Ig (same clone as 66372-1-PBS)

    WB result of PGRMC1 antibody (66372-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PGRMC1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66372-1-PBS in a different storage buffer formulation.

  • WB analysis using 66372-1-Ig (same clone as 66372-1-PBS)

    WB analysis using 66372-1-Ig (same clone as 66372-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66372-1-Ig (PGRMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66372-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 66372-1-Ig (same clone as 66372-1-PBS)

    IF Staining of A549 using 66372-1-Ig (same clone as 66372-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using PGRMC1 antibody (66372-1-Ig, Clone: 1C10D11 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004). This data was developed using the same antibody clone with 66372-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66372-1-Ig (same clone as 66372-1-PBS)

    IHC staining of human ovary tumor using 66372-1-Ig (same clone as 66372-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66372-1-Ig (PGRMC1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66372-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PGRMC1 antibody included
  • IHC staining of human ovary tumor using 66372-1-Ig (same clone as 66372-1-PBS)

    IHC staining of human ovary tumor using 66372-1-Ig (same clone as 66372-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66372-1-Ig (PGRMC1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66372-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PGRMC1 antibody included
  • IF Staining of human ovary tumor using 66372-1-Ig (same clone as 66372-1-PBS)

    IF Staining of human ovary tumor using 66372-1-Ig (same clone as 66372-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using PGRMC1 antibody (66372-1-Ig, Clone: 1C10D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66372-1-PBS in a different storage buffer formulation.

  • IF Staining of human ovary tumor using 66372-1-Ig (same clone as 66372-1-PBS)

    IF Staining of human ovary tumor using 66372-1-Ig (same clone as 66372-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using PGRMC1 antibody (66372-1-Ig, Clone: 1C10D11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66372-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PGRMC1 Monoclonal antibody, PBS Only

PGRMC1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 66372-1-PBS

产品说明书

CloneNo. 1C10D11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

PGRMC, mPR, Membrane-associated progesterone receptor component 1, HPR6.6, 1C10D11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PGRMC1 Ready-To-Use IHC Kit. PGRMC1 primary antibody included.

产品信息

66372-1-PBS targets PGRMC1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 PGRMC1 fusion protein Ag3643 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 progesterone receptor membrane component 1
别名 PGRMC, mPR, Membrane-associated progesterone receptor component 1, HPR6.6, 1C10D11
计算分子量 195 aa, 22 kDa
观测分子量 25-27 kDa
GenBank蛋白编号BC034238
基因名称 PGRMC1
Gene ID (NCBI) 10857
RRIDAB_2881751
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO00264
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Progesterone receptor membrane component 1 (PGRMC1) is a member of a multi-protein progesterone-binding complex. However, PGRMC1 shares homology with cytochrome b5-related proteins rather than hormone receptors (PMID: 18992768). It is a heme binding protein with biding sites for Src homology (SH2) and SH3 domain-containing proteins (PMID: 17583495). PGRMC1 is overexpressed in a variety of cancers, and thus represents an important biomarker for cancer progression and a potential target for anticancer drugs (PMID: 21730960). In nonmalignant tissues, PGRMC1 is highly expressed in the liver and kidney (PMID: 9705155; 20164297).