验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | PC-3 cells |
For other applications, we recommend the unconjugated version of this antibody, 82965-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82965 targets PGM5 in FC (Intra) applications and shows reactivity with human samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human |
| 免疫原 | PGM5 fusion protein Ag34399 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | phosphoglucomutase 5 |
| 别名 | PGMRP, Aciculin, 230206C7 |
| 计算分子量 | 62 kDa |
| 观测分子量 | 62 kDa |
| GenBank蛋白编号 | NM_021965 |
| 基因名称 | PGM5 |
| Gene ID (NCBI) | 5239 |
| RRID | AB_3673156 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q15124 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Phosphoglucomutase-like protein 5 (also known as aciculin) is an enzyme encoded by the PGM5 gene. Gene functional studies show that PGM5 is similar to PGM1 but lacks enzymatic activity. PGM5 is tightly associated with the actin cytoskeleton and has primarily been investigated as an adhesion protein. It also functions as a cytoskeletal component of cell-matrix and cell-cell contacts in muscle and non-muscle cells. (PMID: 35819729)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 PGM5 antibody CL488-82965 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |