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验证数据展示

  • IF Staining of U2OS using CL488-84117

    IF Staining of U2OS using CL488-84117

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 PDLIM4 antibody (CL488-84117, Clone: 241401B3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated PDLIM4 Recombinant antibody

PDLIM4 Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL488-84117

产品说明书

CloneNo. 241401B3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

241401B3, PDZ and LIM domain 4, Reversion-induced LIM protein, RIL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy PDLIM4 Ready-To-Use IHC Kit. PDLIM4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 84117-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-84117 targets PDLIM4 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 PDLIM4 fusion protein Ag13424 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PDZ and LIM domain 4
别名 241401B3, PDZ and LIM domain 4, Reversion-induced LIM protein, RIL
计算分子量 35 kDa
观测分子量35 kDa
GenBank蛋白编号BC016765
基因名称 PDLIM4
Gene ID (NCBI) 8572
RRIDAB_3673342
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP50479
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDLIM4, also known as RIL, is a member of the ALP/Enigma family. PDLIM4 contains one PDZ(DHR) domain and one LIM zinc-binding domain. The LIM zinc-binding domain interacts with the second and fourth PDZ domains of Fap-1, possibly resulting in the dephosphorylation of PDLIM4 (PMID: 21636573). PDLIM4 is involved in osteoblast development and function. The variation of the PDLIM4 locus may be an important determinant of osteoporosis (PMID:12908099).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PDLIM4 antibody CL488-84117Download protocol
Standard Protocols
Click here to view our Standard Protocols