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验证数据展示

  • WB analysis using 84117-1-RR

    WB analysis using 84117-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84117-1-RR (PDLIM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 84117-1-RR

    IF Staining of U2OS using 84117-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PDLIM4 antibody (84117-1-RR, Clone: 241401B3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84117-1-RR

    Affinity and Kinetic Characterization of 84117-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84117-1-RR against Human PDLIM4 were performed. The affinity constant is 2.43 nM.

PDLIM4 Recombinant monoclonal antibody

PDLIM4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84117-1-RR

产品说明书

CloneNo. 241401B3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

RIL, Reversion-induced LIM protein, PDZ and LIM domain 4, 241401B3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, K-562 cells, PC-3 cells
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy PDLIM4 Ready-To-Use IHC Kit. PDLIM4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84117-1-RR targets PDLIM4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag13424

Product name: Recombinant human PDLIM4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-330 aa of BC016765

Sequence: MPHSVTLRGPSPWGFRLVGGRDFSAPLTISRVHAGSKAALAALCPGDLIQAINGESTELMTHLEAQNRIKGCHDHLTLSVSRPEGRSWPSAPDDSKAQAHRIHIDPEIQDGSPTTSRGPSGTGTGPEDGRPSLGSPYGQPPRFPVPHNGSSEATLPAQMSTLHVSPPPSADPARGLPRSRDCRVDLGSEVYRMLREPAEPVAAEPKQSGSFRYLQGMLEAGEGGDWPGPGGPRNLKPTASKLGAPLSGLQGLPECTRCGHGIVGTIVKARDKLYHPECFMCSDCGLNLKQRGYFFLDERLYCESHAKARVKPPEGYDVVAVYPNAKVELV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 PDZ and LIM domain 4
别名 RIL, Reversion-induced LIM protein, PDZ and LIM domain 4, 241401B3
计算分子量 35 kDa
观测分子量35 kDa
GenBank蛋白编号BC016765
基因名称 PDLIM4
Gene ID (NCBI) 8572
RRIDAB_3671681
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP50479
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PDLIM4, also known as RIL, is a member of the ALP/Enigma family. PDLIM4 contains one PDZ(DHR) domain and one LIM zinc-binding domain. The LIM zinc-binding domain interacts with the second and fourth PDZ domains of Fap-1, possibly resulting in the dephosphorylation of PDLIM4 (PMID: 21636573). PDLIM4 is involved in osteoblast development and function. The variation of the PDLIM4 locus may be an important determinant of osteoporosis (PMID:12908099).

实验方案

Product Specific Protocols
IF protocol for PDLIM4 antibody 84117-1-RRDownload protocol
WB protocol for PDLIM4 antibody 84117-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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