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CoraLite® Plus 488-conjugated PAX8 Recombinant antibody

PAX8 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

6B14

Cat No : CL488-80756

Print datasheet

Synonyms

paired box 8, Paired box protein Pax 8, PAX8

验证数据展示

  • IF Staining of SKOV-3 using CL488-80756

    IF Staining of SKOV-3 using CL488-80756

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using CoraLite® Plus 488 PAX8 antibody (CL488-80756, Clone: 6B14 ) at dilution of 1:400.

经过测试的应用

Positive IF detected inSKOV-3 cells
For other applications, we recommend the unconjugated version of this antibody, 80756-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-80756 targets PAX8 in IF applications and shows reactivity with Human, mouse samples.

Tested Applications IF/ICC
Tested Reactivity Human, mouse
Immunogen PAX8 fusion protein Ag0306 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name paired box 8
Synonyms paired box 8, Paired box protein Pax 8, PAX8
Calculated Molecular Weight 48 kDa
Observed Molecular Weight48 kDa, 58 kDa
GenBank Accession NumberBC001060
Gene Symbol PAX8
Gene ID (NCBI) 7849
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06710
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinoma.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PAX8 antibody CL488-80756Download protocol
Standard Protocols
Click here to view our Standard Protocols