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验证数据展示

  • IF Staining of A431 using CL647-68182

    IF Staining of A431 using CL647-68182

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 647 NSFL1C antibody (CL647-68182, Clone: 1F12E12 ) at dilution of 1:200.

  • FC experiment of A431 using CL647-68182

    FC experiment of A431 using CL647-68182

    1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated NSFL1C Monoclonal antibody (CL647-68182, Clone:1F12E12)(red), or 0.4 ug CoraLite® Plus 647 Mouse IgG1 Isotype Control (1F8D3) (CL647-66360-1, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 647-conjugated NSFL1C Monoclonal antibody

NSFL1C Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-68182

产品说明书

CloneNo. 1F12E12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF/ICC, FC (Intra)

别名

UBXN2C, UBX domain-containing protein 2C, p97 cofactor p47, NSFL1 cofactor p47, 1F12E12

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy® NSFL1C Ready-To-Use IHC Kit. NSFL1C primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68182-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-68182 targets NSFL1C in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag8040

Product name: Recombinant human NSFL1C protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-370 aa of BC002801

Sequence: MAAERQEALREFVAVTGAEEDRARFFLESAGWDLQIALASFYEDGGDEDIVTISQATPSSVSRGTAPSDNRVTSFRDLIHDQDEDEEEEEGQRFYAGGSERSGQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVERVTKSPGETSKPRPFAGGGYRLGAAPEEESAYVAGEKRQHSSQDVHVVLKLWKSGFSLDNGELRSYQDPSNAQFLESIRRGEVPAELRRLAHGGQVNLDMEDHRDEDFVKPKGAFKAFTGEGQKLGSTAPQVLSTSSPAQQAENEAKASSSILIDESEPTTNIQIRLADGGRLVQKFNHSHRISDIRLFIVDARPAMAATSFILMTTFPNKELADESQTLKEANLLNAVIVQRLT

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 NSFL1 (p97) cofactor (p47)
别名 UBXN2C, UBX domain-containing protein 2C, p97 cofactor p47, NSFL1 cofactor p47, 1F12E12
计算分子量 41 kDa
观测分子量41 kDa
GenBank蛋白编号BC002801
基因名称 NSFL1C
Gene ID (NCBI) 55968
RRIDAB_3673647
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9UNZ2
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 NSFL1C antibody CL647-68182Download protocol
IF protocol for CL Plus 647 NSFL1C antibody CL647-68182Download protocol
Standard Protocols
Click here to view our Standard Protocols
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