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NSFL1C Monoclonal antibody

NSFL1C Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F12E12

Cat No : 68182-1-Ig

Print datasheet

Synonyms

dJ776F14.1, NSFL1 (p97) cofactor (p47), NSFL1 cofactor p47, NSFL1C, p47, p97 cofactor p47, UBX1, UBXD10, UBXN2C

验证数据展示

  • WB analysis using 68182-1-Ig

    WB analysis using 68182-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68182-1-Ig (NSFL1C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 68182-1-Ig

    WB analysis using 68182-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68182-1-Ig (NSFL1C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human lung cancer using 68182-1-Ig

    IHC staining of human lung cancer using 68182-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68182-1-Ig (NSFL1C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 68182-1-Ig

    IHC staining of human lung cancer using 68182-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68182-1-Ig (NSFL1C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 68182-1-Ig

    IF Staining of A431 using 68182-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NSFL1C antibody (68182-1-Ig, Clone: 1F12E12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inrat brain tissue, U2OS cells, mouse brain tissue, A549 cells, A431 cells, LNCaP cells, HeLa cells, Jurkat cells, pig brain tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 555, CoraLite® Plus 594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68182-1-Ig targets NSFL1C in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen NSFL1C fusion protein Ag8040 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name NSFL1 (p97) cofactor (p47)
Synonyms dJ776F14.1, NSFL1 (p97) cofactor (p47), NSFL1 cofactor p47, NSFL1C, p47, p97 cofactor p47, UBX1, UBXD10, UBXN2C
Calculated Molecular Weight 41 kDa
Observed Molecular Weight41 kDa
GenBank Accession NumberBC002801
Gene Symbol NSFL1C
Gene ID (NCBI) 55968
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UNZ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NSFL1C antibody 68182-1-IgDownload protocol
IHC protocol for NSFL1C antibody 68182-1-IgDownload protocol
IF protocol for NSFL1C antibody 68182-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols