验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy® NSFL1C Ready-To-Use IHC Kit. NSFL1C primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68182-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL555-68182 targets NSFL1C in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human, Mouse, Rat, Pig |
| 免疫原 |
CatNo: Ag8040 Product name: Recombinant human NSFL1C protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-370 aa of BC002801 Sequence: MAAERQEALREFVAVTGAEEDRARFFLESAGWDLQIALASFYEDGGDEDIVTISQATPSSVSRGTAPSDNRVTSFRDLIHDQDEDEEEEEGQRFYAGGSERSGQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVERVTKSPGETSKPRPFAGGGYRLGAAPEEESAYVAGEKRQHSSQDVHVVLKLWKSGFSLDNGELRSYQDPSNAQFLESIRRGEVPAELRRLAHGGQVNLDMEDHRDEDFVKPKGAFKAFTGEGQKLGSTAPQVLSTSSPAQQAENEAKASSSILIDESEPTTNIQIRLADGGRLVQKFNHSHRISDIRLFIVDARPAMAATSFILMTTFPNKELADESQTLKEANLLNAVIVQRLT 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | NSFL1 (p97) cofactor (p47) |
| 别名 | dJ776F14.1, NSFL1 (p97) cofactor (p47), NSFL1 cofactor p47, NSFL1C, p47, p97 cofactor p47, UBX1, UBXD10, UBXN2C |
| 计算分子量 | 41 kDa |
| 观测分子量 | 41 kDa |
| GenBank蛋白编号 | BC002801 |
| 基因名称 | NSFL1C |
| Gene ID (NCBI) | 55968 |
| RRID | AB_3084635 |
| 偶联类型 | CoraLite® Plus 555 Fluorescent Dye |
| 最大激发/发射波长 | 554 nm / 570 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9UNZ2 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 555 NSFL1C antibody CL555-68182 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
