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验证数据展示

  • IF Staining of U2OS using CL488-82879

    IF Staining of U2OS using CL488-82879

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite® Plus 488 NOP58 antibody (CL488-82879, Clone: 230058C7 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-82879

    FC experiment of HeLa using CL488-82879

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated NOP58 Recombinant antibody (CL488-82879, Clone:230058C7)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated NOP58 Recombinant monoclonal antibody

NOP58 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-82879

产品说明书

CloneNo. 230058C7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

Nucleolar protein 5, NOP5/NOP58, NOP5, NOL5, HSPC120

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NOP58 Ready-To-Use IHC Kit. NOP58 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82879-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-82879 targets NOP58 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag5740

Product name: Recombinant human NOP58 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 279-529 aa of BC032592

Sequence: MMAIAPNVTVMVGELVGARLIAHAGSLLNLAKHAASTVQILGAEKALFRALKSRRDTPKYGLIYHASLVGQTSPKHKGKISRMLAAKTVLAIRYDAFGEDSSSAMGVENRAKLEARLRTLEDRGIRKISGTGKALAKTEKYEHKSEVKTYDPSGDSTLPTCSKKRKIEQVDKEDEITEKKAKKAKIKVKVEEEEEEKVAEEEETSVKKKKKRGKKKHIKEEPLSEEEPCTSTAIASPEKKKKKKKKRENED

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NOP58 ribonucleoprotein homolog (yeast)
别名 Nucleolar protein 5, NOP5/NOP58, NOP5, NOL5, HSPC120
计算分子量 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC032592
基因名称 NOP58
Gene ID (NCBI) 51602
RRIDAB_3673094
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y2X3
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The box C/D snoRNPs are named for the short, conserved box C (UGAUGA) and box D (CUGA) sequences present in their RNA moiety. The box C/D sequences are also required for multiple aspects. of snoRNA biogenesis, including RNA stability, intronic processing, nucleolar targeting, nuclear retention, and 59 trimethylguanosine cap formation (PMID:9649444,8878486). Nop58 is a component common to the box C/D small nucleolar ribonucleoprotein (PMID:10606270).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 NOP58 antibody CL488-82879Download protocol
IF protocol for CL Plus 488 NOP58 antibody CL488-82879Download protocol
Standard Protocols
Click here to view our Standard Protocols
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