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NOP58 Recombinant antibody

NOP58 Recombinant Antibody for WB, IP, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230058C7

Cat No : 82879-1-RR

Print datasheet

Synonyms

HSPC120, NOL5, NOP5, NOP5/NOP58, NOP58, Nucleolar protein 5, Nucleolar protein 58

验证数据展示

  • WB analysis using 82879-1-RR

    WB analysis using 82879-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82879-1-RR (NOP58 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 82879-1-RR

    IP experiment of HeLa using 82879-1-RR

    IP result of anti-NOP58 (IP:82879-1-RR, 4ug; Detection:82879-1-RR 1:2000) with HeLa cells lysate 1600 ug.

  • IHC staining of human prostate cancer using 82879-1-RR

    IHC staining of human prostate cancer using 82879-1-RR

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82879-1-RR (NOP58 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NOP58 antibody included
  • IF Staining of U2OS using 82879-1-RR

    IF Staining of U2OS using 82879-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NOP58 antibody (82879-1-RR) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, MCF-7 cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy NOP58 Ready-To-Use IHC Kit. NOP58 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82879-1-RR targets NOP58 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IP, IF/ICC, IHC, ELISA
Tested Reactivity Human
Immunogen NOP58 fusion protein Ag5740 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name NOP58 ribonucleoprotein homolog (yeast)
Synonyms HSPC120, NOL5, NOP5, NOP5/NOP58, NOP58, Nucleolar protein 5, Nucleolar protein 58
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC032592
Gene Symbol NOP58
Gene ID (NCBI) 51602
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y2X3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The box C/D snoRNPs are named for the short, conserved box C (UGAUGA) and box D (CUGA) sequences present in their RNA moiety. The box C/D sequences are also required for multiple aspects. of snoRNA biogenesis, including RNA stability, intronic processing, nucleolar targeting, nuclear retention, and 59 trimethylguanosine cap formation (PMID:9649444,8878486). Nop58 is a component common to the box C/D small nucleolar ribonucleoprotein (PMID:10606270).

实验方案

Product Specific Protocols
WB protocol for NOP58 antibody 82879-1-RRDownload protocol
IHC protocol for NOP58 antibody 82879-1-RRDownload protocol
IF protocol for NOP58 antibody 82879-1-RRDownload protocol
IP protocol for NOP58 antibody 82879-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols