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验证数据展示

  • WB analysis using 82879-1-RR (same clone as 82879-1-PBS)

    WB analysis using 82879-1-RR (same clone as 82879-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82879-1-RR (NOP58 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82879-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 82879-1-RR (same clone as 82879-1-PBS)

    IP experiment of HeLa using 82879-1-RR (same clone as 82879-1-PBS)

    IP result of anti-NOP58 (IP:82879-1-RR, 4ug; Detection:82879-1-RR 1:2000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 82879-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 82879-1-RR (same clone as 82879-1-PBS)

    WB analysis of MCF-7 using 82879-1-RR (same clone as 82879-1-PBS)

    WB result of NOP58 antibody (82879-1-RR; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NOP58 transfected MCF-7 cells. This data was developed using the same antibody clone with 82879-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 82879-1-RR (same clone as 82879-1-PBS)

    IHC staining of human prostate cancer using 82879-1-RR (same clone as 82879-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82879-1-RR (NOP58 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82879-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NOP58 antibody included
  • IF Staining of U2OS using 82879-1-RR (same clone as 82879-1-PBS)

    IF Staining of U2OS using 82879-1-RR (same clone as 82879-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NOP58 antibody (82879-1-RR) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82879-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82879-1-RR (same clone as 82879-1-PBS)

    FC experiment of HeLa using 82879-1-RR (same clone as 82879-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NOP58 Recombinant antibody (82879-1-RR, Clone:230058C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82879-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82879-1-RR

    Affinity and Kinetic Characterization of 82879-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82879-1-RR against Human NOP58 were performed. The affinity constant is 5.53 nM.

  • Affinity and Kinetic Characterization of 82879-1-RR

    Affinity and Kinetic Characterization of 82879-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82879-1-RR against Human NOP58 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

NOP58 Recombinant monoclonal antibody, PBS Only

NOP58 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 82879-1-PBS

产品说明书

CloneNo. 230058C7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

230058C7, HSPC120, NOL5, NOP5, NOP5/NOP58

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy NOP58 Ready-To-Use IHC Kit. NOP58 primary antibody included.

产品信息

82879-1-PBS targets NOP58 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5740

Product name: Recombinant human NOP58 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 279-529 aa of BC032592

Sequence: MMAIAPNVTVMVGELVGARLIAHAGSLLNLAKHAASTVQILGAEKALFRALKSRRDTPKYGLIYHASLVGQTSPKHKGKISRMLAAKTVLAIRYDAFGEDSSSAMGVENRAKLEARLRTLEDRGIRKISGTGKALAKTEKYEHKSEVKTYDPSGDSTLPTCSKKRKIEQVDKEDEITEKKAKKAKIKVKVEEEEEEKVAEEEETSVKKKKKRGKKKHIKEEPLSEEEPCTSTAIASPEKKKKKKKKRENED

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 NOP58 ribonucleoprotein homolog (yeast)
别名 230058C7, HSPC120, NOL5, NOP5, NOP5/NOP58
计算分子量 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC032592
基因名称 NOP58
Gene ID (NCBI) 51602
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y2X3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The box C/D snoRNPs are named for the short, conserved box C (UGAUGA) and box D (CUGA) sequences present in their RNA moiety. The box C/D sequences are also required for multiple aspects. of snoRNA biogenesis, including RNA stability, intronic processing, nucleolar targeting, nuclear retention, and 59 trimethylguanosine cap formation (PMID:9649444,8878486). Nop58 is a component common to the box C/D small nucleolar ribonucleoprotein (PMID:10606270).

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