验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, pig brain tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human colon cancer tissue |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 1 publications below |
产品信息
66253-1-Ig targets GNAS in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, pig samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, pig |
| 文献引用反应性 | human, pig |
| 免疫原 |
CatNo: Ag20530 Product name: Recombinant human NESP55,GNAS protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 230-394 aa of BC002722 Sequence: ERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | GNAS complex locus |
| 别名 | 1A9C10, GNAS1, GPIPIRRH peptide, LHAL tetrapeptide, NESP55 |
| 计算分子量 | 45 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC002722 |
| 基因名称 | GNAS |
| Gene ID (NCBI) | 2778 |
| RRID | AB_2881641 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P63092 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for GNAS antibody 66253-1-Ig | Download protocol |
| IHC protocol for GNAS antibody 66253-1-Ig | Download protocol |
| WB protocol for GNAS antibody 66253-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Vet Microbiol Porcine circovirus-like virus P1 activates pancreatic secretion pathway by interacting with CHRM3 protein | ||
FASEB J GRP78 acts as a cAMP/PKA signaling modulator through the MC4R pathway in porcine embryonic development | ||
iScience Bitter taste receptor T2R14-Gαi coupling mediates innate immune responses to microbial quorum sensing molecules in cystic fibrosis | ||
Cancer Lett Cancer Associated Fibroblasts-derived Lactate Induces Oxaliplatin Treatment Resistance by Promoting Cancer Stemness via ANTXR1 Lactylation in Colorectal Cancer |
