验证数据展示
经过测试的应用
| Positive IF-P detected in | human colon cancer tissue |
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy GNAS Ready-To-Use IHC Kit. GNAS primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66253-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66253 targets GNAS in IF/ICC, IF-P applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC, IF-P Application Description |
| 经测试反应性 | human |
| 免疫原 | GNAS fusion protein Ag20530 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | GNAS complex locus |
| 别名 | Neuroendocrine secretory protein 55, NESP55, LHAL tetrapeptide, GPIPIRRH peptide, GNAS1 |
| 计算分子量 | 45 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC002722 |
| 基因名称 | GNAS |
| Gene ID (NCBI) | 2778 |
| RRID | AB_2919971 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O95467 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 GNAS antibody CL594-66253 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
