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CoraLite® Plus 488-conjugated GNAS Monoclonal antibody

GNAS Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A9C10

Cat No : CL488-66253

Print datasheet

Synonyms

AHO, C20orf45, dJ309F20.1.1, dJ806M20.3.3, GNAS, GNAS complex locus, GNAS1, GPSA, GSA, GSP, NESP, NESP55, PHP1A, PHP1B, POH, Protein ALEX

验证数据展示

  • IF Staining of MCF-7 using CL488-66253

    IF Staining of MCF-7 using CL488-66253

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 GNAS antibody (CL488-66253, Clone: 1A9C10 ) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-66253

    FC experiment of MCF-7 using CL488-66253

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human GNAS (CL488-66253, Clone:1A9C10) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66253-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66253 targets GNAS in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human
Immunogen GNAS fusion protein Ag20530 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name GNAS complex locus
Synonyms AHO, C20orf45, dJ309F20.1.1, dJ806M20.3.3, GNAS, GNAS complex locus, GNAS1, GPSA, GSA, GSP, NESP, NESP55, PHP1A, PHP1B, POH, Protein ALEX
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC002722
Gene Symbol GNAS
Gene ID (NCBI) 2778
RRIDAB_2919305
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95467
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GNAS antibody CL488-66253Download protocol
FC protocol for CL Plus 488 GNAS antibody CL488-66253Download protocol
Standard Protocols
Click here to view our Standard Protocols