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验证数据展示

  • WB analysis of fetal human brain using 66253-1-Ig (same clone as 66253-1-PBS)

    WB analysis of fetal human brain using 66253-1-Ig (same clone as 66253-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66253-1-Ig (NESP55,GNAS Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66253-1-Ig (same clone as 66253-1-PBS)

    IHC staining of human colon using 66253-1-Ig (same clone as 66253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66253-1-Ig (GNAS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GNAS antibody included
  • IHC staining of human colon using 66253-1-Ig (same clone as 66253-1-PBS)

    IHC staining of human colon using 66253-1-Ig (same clone as 66253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66253-1-Ig (GNAS antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GNAS antibody included
  • IF Staining of MCF-7 using 66253-1-Ig (same clone as 66253-1-PBS)

    IF Staining of MCF-7 using 66253-1-Ig (same clone as 66253-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 66253-1-Ig (same clone as 66253-1-PBS)

    IF Staining of human colon cancer using 66253-1-Ig (same clone as 66253-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

GNAS Monoclonal antibody, PBS Only

GNAS Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 66253-1-PBS

产品说明书

CloneNo. 1A9C10

宿主/亚型

Mouse / IgG2b

种属反应性

human, pig

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

1A9C10, GNAS1, GPIPIRRH peptide, LHAL tetrapeptide, NESP55

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GNAS Ready-To-Use IHC Kit. GNAS primary antibody included.

产品信息

66253-1-PBS targets GNAS in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag20530

Product name: Recombinant human NESP55,GNAS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 230-394 aa of BC002722

Sequence: ERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 GNAS complex locus
别名 1A9C10, GNAS1, GPIPIRRH peptide, LHAL tetrapeptide, NESP55
计算分子量45 kDa
观测分子量 46 kDa
GenBank蛋白编号BC002722
基因名称 GNAS
Gene ID (NCBI) 2778
RRIDAB_2881641
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP63092
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.

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