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验证数据展示

  • WB analysis of fetal human brain using 66253-1-Ig (same clone as 66253-1-PBS)

    WB analysis of fetal human brain using 66253-1-Ig (same clone as 66253-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66253-1-Ig (NESP55,GNAS Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66253-1-Ig (same clone as 66253-1-PBS)

    IHC staining of human colon using 66253-1-Ig (same clone as 66253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66253-1-Ig (GNAS antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GNAS antibody included
  • IHC staining of human colon using 66253-1-Ig (same clone as 66253-1-PBS)

    IHC staining of human colon using 66253-1-Ig (same clone as 66253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66253-1-Ig (GNAS antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GNAS antibody included
  • IF Staining of MCF-7 using 66253-1-Ig (same clone as 66253-1-PBS)

    IF Staining of MCF-7 using 66253-1-Ig (same clone as 66253-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

  • IF Staining of human colon cancer using 66253-1-Ig (same clone as 66253-1-PBS)

    IF Staining of human colon cancer using 66253-1-Ig (same clone as 66253-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using GNAS antibody (66253-1-Ig, Clone: 1A9C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66253-1-PBS in a different storage buffer formulation.

GNAS Monoclonal antibody, PBS Only

GNAS Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 66253-1-PBS

产品说明书

CloneNo. 1A9C10

宿主/亚型

Mouse / IgG2b

种属反应性

human, pig

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

Neuroendocrine secretory protein 55, NESP55, LHAL tetrapeptide, GPIPIRRH peptide, GNAS1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GNAS Ready-To-Use IHC Kit. GNAS primary antibody included.

产品信息

66253-1-PBS targets GNAS in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag20530

Product name: Recombinant human NESP55,GNAS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 230-394 aa of BC002722

Sequence: ERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 GNAS complex locus
别名 Neuroendocrine secretory protein 55, NESP55, LHAL tetrapeptide, GPIPIRRH peptide, GNAS1
计算分子量 45 kDa
观测分子量 46 kDa
GenBank蛋白编号BC002722
基因名称 GNAS
Gene ID (NCBI) 2778
RRIDAB_2881641
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95467
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.

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