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CoraLite®594-conjugated Myosin Light Chain 2 Monoclonal antibody

Myosin Light Chain 2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5A1F1

Cat No : CL594-60229

Print datasheet

Synonyms

CMH10, DKFZp779C0562, MLC 2, MLC 2v, MLC2, MLC2v, MYL2

验证数据展示

  • IF Staining of C2C12 using CL594-60229

    IF Staining of C2C12 using CL594-60229

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CL594-60229 (Myosin Light Chain 2 antibody) at dilution of 1:50. .

经过测试的应用

Positive IF detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy MYL2 Ready-To-Use IHC Kit. MYL2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60229-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL594-60229 is Myosin Light Chain 2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC
Tested Reactivity human, mouse, rat, pig
Immunogen Myosin Light Chain 2 fusion protein Ag1356 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name myosin, light chain 2, regulatory, cardiac, slow
Synonyms CMH10, DKFZp779C0562, MLC 2, MLC 2v, MLC2, MLC2v, MYL2
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC015821
Gene Symbol MYL2
Gene ID (NCBI) 4633
RRIDAB_2883447
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP10916
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.

实验方案

Product Specific Protocols
IF protocol for CL594 Myosin Light Chain 2 antibody CL594-60229Download protocol
Standard Protocols
Click here to view our Standard Protocols