Myosin Light Chain 2/MLC-2V Monoclonal antibody

Myosin Light Chain 2/MLC-2V Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60229-1-Ig

产品说明书

CloneNo. 5A1F1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, ELISA and More (1)

MLC 2v, MYL2, MLC-2s/v, MLC-2, MLC2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inhuman heart tissue, pig heart tissue, rat heart tissue, rabbit heart tissue
Positive IHC detected inmouse heart tissue, human heart tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy MYL2 Ready-To-Use IHC Kit. MYL2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60229-1-Ig targets Myosin Light Chain 2/MLC-2V in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat, pig, rabbit
文献引用反应性human, rat
免疫原 Myosin Light Chain 2/MLC-2V fusion protein Ag1356 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 myosin, light chain 2, regulatory, cardiac, slow
别名 MLC 2v, MYL2, MLC-2s/v, MLC-2, MLC2
计算分子量 19 kDa
观测分子量 18 kDa
GenBank蛋白编号BC015821
基因名称 Myosin Light Chain 2
Gene ID (NCBI) 4633
RRIDAB_2881356
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP10916
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.

实验方案

Product Specific Protocols
WB protocol for Myosin Light Chain 2/MLC-2V antibody 60229-1-IgDownload protocol
IHC protocol for Myosin Light Chain 2/MLC-2V antibody 60229-1-IgDownload protocol
IF protocol for Myosin Light Chain 2/MLC-2V antibody 60229-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Stem Cells

Nitric Oxide-cGMP-PKG Pathway Acts on Orai1 to Inhibit the Hypertrophy of Human Embryonic Stem Cell-Derived Cardiomyocytes.

Authors - Y Wang
ratWB

Int J Stem Cells

Synergistic Effect of Hydrogen and 5-Aza on Myogenic Differentiation through the p38 MAPK Signaling Pathway in Adipose-Derived Mesenchymal Stem Cells

Authors - Jingcheng Wang
humanIF

Stem Cell Rev Rep

MYOCD is Required for Cardiomyocyte-like Cells Induction from Human Urine Cells and Fibroblasts Through Remodeling Chromatin.

Authors - Xiangyu Zhang
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