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验证数据展示

  • IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)

    IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:500 (under 0x lens). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYL2 antibody included
  • IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)

    IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:500 (under 0x lens). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYL2 antibody included
  • WB analysis using 60229-1-Ig (same clone as 60229-1-PBS)

    WB analysis using 60229-1-Ig (same clone as 60229-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 60229-1-Ig (same clone as 60229-1-PBS)

    IHC staining of mouse skeletal muscle using 60229-1-Ig (same clone as 60229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYL2 antibody included
  • IHC staining of mouse heart using 60229-1-Ig (same clone as 60229-1-PBS)

    IHC staining of mouse heart using 60229-1-Ig (same clone as 60229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60229-1-Ig (Myosin Light Chain 2/MLC-2V antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYL2 antibody included
  • IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)

    IHC staining of human heart using 60229-1-Ig (same clone as 60229-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60229-1-Ig (Myosin Light Chain 2 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MYL2 antibody included
  • IF Staining of C2C12 using 60229-1-Ig (same clone as 60229-1-PBS)

    IF Staining of C2C12 using 60229-1-Ig (same clone as 60229-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using Myosin Light Chain 2/MLC-2V antibody (60229-1-Ig, Clone: 5A1F1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60229-1-PBS in a different storage buffer formulation.

Myosin Light Chain 2/MLC-2V Monoclonal antibody, PBS Only

Myosin Light Chain 2/MLC-2V Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60229-1-PBS

产品说明书

CloneNo. 5A1F1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

MLC 2v, MYL2, MLC-2s/v, MLC-2, MLC2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MYL2 Ready-To-Use IHC Kit. MYL2 primary antibody included.

产品信息

60229-1-PBS targets Myosin Light Chain 2/MLC-2V in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag1356

Product name: Recombinant human MYL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-166 aa of BC015821

Sequence: MAPKKAKKRAGGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKNDLRDTFAALGRVNVKNEEIDEMIKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGKGVLKADYVREMLTTQAERFSKEEVDQMFAAFPPDVTGNLDYKNLVHIITHGEEKD

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 myosin, light chain 2, regulatory, cardiac, slow
别名 MLC 2v, MYL2, MLC-2s/v, MLC-2, MLC2
计算分子量 19 kDa
观测分子量 18 kDa
GenBank蛋白编号BC015821
基因名称 Myosin Light Chain 2
Gene ID (NCBI) 4633
RRIDAB_2881356
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP10916
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MYL2, also named as MLC-2v and MLC-2, is ventricular/cardiac muscle isoform. Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10). Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic with mid-left ventricular chamber type 2 (MVC2). MYL2 has been widely used as a marker of mature ventricular cardiomyocytes.

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