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验证数据展示

  • IF Staining of mouse skeletal muscle using CL594-67299

    IF Staining of mouse skeletal muscle using CL594-67299

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CoraLite®594 MYH1 antibody (CL594-67299, Clone: 1G10H9 ) at dilution of 1:200.

CoraLite®594-conjugated MYH1 Monoclonal antibody

MYH1 Monoclonal Antibody for IF-P
Cat No. CL594-67299

产品说明书

CloneNo. 1G10H9

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, pig

应用

IF-P

别名

MYH1, MYHa, MyHC 2x, MyHC 2X/D, MyHC IIx/d, MYHSA1, Myosin 1, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy MYH1 Ready-To-Use IHC Kit. MYH1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67299-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67299 targets MYH1 in IF-P applications and shows reactivity with Human, mouse, pig samples.

经测试应用 IF-P Application Description
经测试反应性 Human, mouse, pig
免疫原

CatNo: Ag17129

Product name: Recombinant human MYH1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC114545

Sequence: MSSDSEMAIFGEAAPFLRKSERERIEAQNKPFDAKTSVFVVDPKESFVKATVQSREGGKVTAKTEAGATVTVKDDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEVTSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQIMSNKKPDLIEMLLITTNPYDYAFVSQGEITVPSIDDQEELMATDSAIEILGFTSDERV

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 myosin, heavy chain 1, skeletal muscle, adult
别名 MYH1, MYHa, MyHC 2x, MyHC 2X/D, MyHC IIx/d, MYHSA1, Myosin 1, Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d
计算分子量 1939 aa, 223 kDa
观测分子量 220 kDa
GenBank蛋白编号BC114545
基因名称 MYH1
Gene ID (NCBI) 4619
RRIDAB_2934763
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP12882
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2.

实验方案

Product Specific Protocols
IF protocol for CL594 MYH1 antibody CL594-67299Download protocol
Standard Protocols
Click here to view our Standard Protocols
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