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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated MMP1 Polyclonal antibody

MMP1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL647-10371

Print datasheet

Synonyms

CLG, CLGN, Fibroblast collagenase, Interstitial collagenase, Matrix metalloproteinase 1, MMP 1, MMP1

验证数据展示

  • FC experiment of HepG2 using CL647-10371

    FC experiment of HepG2 using CL647-10371

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human MMP1 (CL647-10371) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MMP1 Ready-To-Use IHC Kit. MMP1 primary antibody included.

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10371 targets MMP1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity human, mouse, rat
Immunogen MMP1 fusion protein Ag0547 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name matrix metallopeptidase 1 (interstitial collagenase)
Synonyms CLG, CLGN, Fibroblast collagenase, Interstitial collagenase, Matrix metalloproteinase 1, MMP 1, MMP1
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 52-56 kDa, 62 kDa
GenBank Accession NumberBC013875
Gene Symbol MMP1
Gene ID (NCBI) 4312
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP03956
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MMP1, also named as CLG, belongs to the peptidase M10A family. MMP is the main enzyme that cleaves fibrillar collagen, namely types I, II, III, VII, and X.It is involved in cell migration and invasion, and is frequently up-regulated in cancer cells. It can be cleavage to two major forms (22 kDa and 27 kDa) by undergoing autolytic,and the minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.The sizes of pro-MMP-1 and active MMP-1 from human synovial fibroblasts have been reported as 52 to 56 kDa, depending on glycosylation, and 41 to 45 kDa, respectively.In addition ,a band of 62-kDa observed in the current study is pro-MMp-1(PMID:9418730).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 MMP1 antibody CL647-10371Download protocol
Standard Protocols
Click here to view our Standard Protocols