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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated MIF Polyclonal antibody

MIF Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL647-20415

Print datasheet

Synonyms

GIF, GLIF, L dopachrome isomerase, L dopachrome tautomerase, MIF, MMIF, Phenylpyruvate tautomerase

验证数据展示

  • FC experiment of A549 using CL647-20415

    FC experiment of A549 using CL647-20415

    1X10^6 A549 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human MIF (CL647-20415) (red), or 0.2 ug CL647-30000 Rabbit IgG (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 20415-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL647-20415 is MIF Fusion Protein expressed in E. coli.

Tested Applications FC (Intra)
Tested Reactivity human, rat, mouse
Immunogen MIF fusion protein Ag14058 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Synonyms GIF, GLIF, L dopachrome isomerase, L dopachrome tautomerase, MIF, MMIF, Phenylpyruvate tautomerase
Calculated Molecular Weight 115 aa, 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC000447
Gene Symbol MIF
Gene ID (NCBI) 4282
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14174
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 MIF antibody CL647-20415Download protocol
Standard Protocols
Click here to view our Standard Protocols