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验证数据展示

  • WB analysis using 82695-4-RR (same clone as 82695-4-PBS)

    WB analysis using 82695-4-RR (same clone as 82695-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82695-4-RR (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82695-4-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 82695-4-RR (same clone as 82695-4-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 82695-4-RR (same clone as 82695-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82695-4-RR (MCL1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82695-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MCL1 antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 82695-4-RR (same clone as 82695-4-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 82695-4-RR (same clone as 82695-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82695-4-RR (MCL1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82695-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MCL1 antibody included
  • IF Staining of MCF-7 using 82695-4-RR (same clone as 82695-4-PBS)

    IF Staining of MCF-7 using 82695-4-RR (same clone as 82695-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCL1 antibody (82695-4-RR, Clone: 5P13 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82695-4-PBS in a different storage buffer formulation.

  • FC experiment of Ramos using 82695-4-RR (same clone as 82695-4-PBS)

    FC experiment of Ramos using 82695-4-RR (same clone as 82695-4-PBS)

    1x10^6 Ramos cells were intracellularly stained with 0.25 ug MCL1 Recombinant antibody (82695-4-RR, Clone:5P13) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82695-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82695-4-RR

    Affinity and Kinetic Characterization of 82695-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82695-4-RR against Human MCL1 were performed. The affinity constant is 2.13 nM.

MCL1 Recombinant monoclonal antibody, PBS Only

MCL1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82695-4-PBS

产品说明书

CloneNo. 5P13

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

5P13, Bcl2-L-3, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, EAT

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MCL1 Ready-To-Use IHC Kit. MCL1 primary antibody included.

产品信息

82695-4-PBS targets MCL1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10397

Product name: Recombinant human MCL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-327 aa of BC107735

Sequence: MFGLKRNAVIGLNLYCGGAGLGAGSGGATRPGGRLLATEKEASARREIGGGEAGAVIGGSAGASPPSTLTPDSRRVARPPPIGAEVPDVTATPARLLFFAPTRRAAPLEEMEAPAADAIMSPEEELDGYEPEPLGKRPAVLPLLELVGESGNNTSTDGSLPSTPPPAEEEEDELYRQSLEIISRYLREQATGAKDTKPMGRSGATSRKALETLRRVGDGVQRNHETAFQGMLRKLDIKNEDDVKSLSRVMIHVFSDGVTNWGRIVTLISFGAFVAKHLKTINQESCIEPLAESITDVLVRTKRDWLVKQRGWDGFVEFFHVEDLEGG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 myeloid cell leukemia sequence 1 (BCL2-related)
别名 5P13, Bcl2-L-3, Bcl-2-like protein 3, Bcl-2-related protein EAT/mcl1, EAT
计算分子量 350 aa, 37 kDa
观测分子量40 kDa
GenBank蛋白编号BC107735
基因名称 MCL1
Gene ID (NCBI) 4170
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ07820
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumor malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target. MCL-1 can be alternatively spliced into a long form (MCL-1L, 40 kDa) or a short form (MCL-1S, 30 kDa). (PMID: 15467463, PMID: 15147382)

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