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验证数据展示

  • WB analysis using 85186-3-RR (same clone as 85186-3-PBS)

    WB analysis using 85186-3-RR (same clone as 85186-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85186-3-RR (MAFF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85186-3-PBS in a different storage buffer formulation.

  • WB analysis using 85186-3-RR (same clone as 85186-3-PBS)

    WB analysis using 85186-3-RR (same clone as 85186-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85186-3-RR (MAFF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85186-3-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 85186-3-RR (same clone as 85186-3-PBS)

    IHC staining of human lung cancer using 85186-3-RR (same clone as 85186-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85186-3-RR (MAFF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85186-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAFF antibody included
  • IHC staining of human lung cancer using 85186-3-RR (same clone as 85186-3-PBS)

    IHC staining of human lung cancer using 85186-3-RR (same clone as 85186-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85186-3-RR (MAFF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85186-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAFF antibody included
  • IHC staining of human ovary cancer using 85186-3-RR (same clone as 85186-3-PBS)

    IHC staining of human ovary cancer using 85186-3-RR (same clone as 85186-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85186-3-RR (MAFF antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85186-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with MAFF antibody included
  • IF Staining of U2OS using 85186-3-RR (same clone as 85186-3-PBS)

    IF Staining of U2OS using 85186-3-RR (same clone as 85186-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MAFF antibody (85186-3-RR, Clone: 242859F9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85186-3-PBS in a different storage buffer formulation.

MAFF Recombinant monoclonal antibody, PBS Only

MAFF Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85186-3-PBS

产品说明书

CloneNo. 242859F9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

242859F9, U MAF, U-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog F

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy MAFF Ready-To-Use IHC Kit. MAFF primary antibody included.

产品信息

85186-3-PBS targets MAFF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3460

Product name: Recombinant human MAFF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-164 aa of BC015037

Sequence: MSVDPLSSKALKIKRELSENTPHLSDEALMGLSVRELNRHLRGLSAEEVTRLKQRRRTLKNRGYAASCRVKRVCQKEELQKQKSELEREVDKLARENAAMRLELDALRGKCEALQGFARSVAAARGPATLVAPASVITIVKSTPGSGSGPAHGPDPAHGPASCS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian)
别名 242859F9, U MAF, U-Maf, V-maf musculoaponeurotic fibrosarcoma oncogene homolog F
计算分子量 17 aa, 8 kDa
观测分子量 18-23 kDa
GenBank蛋白编号BC015037
基因名称 MAFF
Gene ID (NCBI) 23764
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9ULX9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MAFF encodes a basic region and leucine zipper (bZIP)-type transcription factor protein (PMID: 8361754). MAFF interacts with the upstream promoter region of oxytocin receptor (OXTR) and plays a role in OXTR upregulation (PubMed: 7798245, PubMed:10527846). Mice lacking MAFF, MAFG and MAFK are embryonic lethal (PMID: 22158967). MAFF has a higher expression in adipose, colon, lung, prostate and skeletal muscle tissues (PMID: 24304889).

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