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验证数据展示

  • WB analysis using 85186-3-RR

    WB analysis using 85186-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85186-3-RR (MAFF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 85186-3-RR

    WB analysis using 85186-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85186-3-RR (MAFF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 85186-3-RR

    IF Staining of U2OS using 85186-3-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MAFF antibody (85186-3-RR, Clone: 242859F9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

MAFF Recombinant antibody

MAFF Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85186-3-RR

产品说明书

CloneNo. 242859F9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

V-maf musculoaponeurotic fibrosarcoma oncogene homolog F, U-Maf, U MAF, 242859F9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, mouse placenta tissue, HuH-7 cells, L02 cells, 4T1 cells
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy MAFF Ready-To-Use IHC Kit. MAFF primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85186-3-RR targets MAFF in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 MAFF fusion protein Ag3460 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-maf musculoaponeurotic fibrosarcoma oncogene homolog F (avian)
别名 V-maf musculoaponeurotic fibrosarcoma oncogene homolog F, U-Maf, U MAF, 242859F9
计算分子量 17 aa, 8 kDa
观测分子量 18-23 kDa
GenBank蛋白编号BC015037
基因名称 MAFF
Gene ID (NCBI) 23764
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9ULX9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAFF encodes a basic region and leucine zipper (bZIP)-type transcription factor protein (PMID: 8361754). MAFF interacts with the upstream promoter region of oxytocin receptor (OXTR) and plays a role in OXTR upregulation (PubMed: 7798245, PubMed:10527846). Mice lacking MAFF, MAFG and MAFK are embryonic lethal (PMID: 22158967). MAFF has a higher expression in adipose, colon, lung, prostate and skeletal muscle tissues (PMID: 24304889).

实验方案

Product Specific Protocols
WB protocol for MAFF antibody 85186-3-RRDownload protocol
IF protocol for MAFF antibody 85186-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols