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CoraLite®594-conjugated LONP1 Monoclonal antibody

LONP1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C6C12

Cat No : CL594-66043

Print datasheet

Synonyms

hLON, LON, lon peptidase 1, mitochondrial, Lon protease like protein, LonHS, LONP, LONP1, PIM1, PRSS15, Serine protease 15

验证数据展示

  • IF Staining of C6 using CL594-66043

    IF Staining of C6 using CL594-66043

    Immunofluorescent analysis of (4% PFA) fixed C6 cells using CoraLite®594 LONP1 antibody (CL594-66043, Clone: 1C6C12 ) at dilution of 1:100. DAPI (blue).

  • IF Staining of C6 using CL594-66043

    IF Staining of C6 using CL594-66043

    Immunofluorescent analysis of (4% PFA) fixed C6 cells using CoraLite®594 LONP1 antibody (CL594-66043, Clone: 1C6C12 ) at dilution of 1:100, CL488-phalloidine (green).

  • IF Staining of C6 using CL594-66043

    IF Staining of C6 using CL594-66043

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using CoraLite®594 LONP1 antibody (CL594-66043, Clone: 1C6C12 ) at dilution of 1:100, CL488-phalloidine (green).

经过测试的应用

Positive IF detected inC6 cells
For other applications, we recommend the unconjugated version of this antibody, 66043-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL594-66043 is LONP1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC
Tested Reactivity human, mouse, rat
Immunogen LONP1 fusion protein Ag7306 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lon peptidase 1, mitochondrial
Synonyms hLON, LON, lon peptidase 1, mitochondrial, Lon protease like protein, LonHS, LONP, LONP1, PIM1, PRSS15, Serine protease 15
Calculated Molecular Weight 106 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC000235
Gene Symbol LONP1
Gene ID (NCBI) 9361
RRIDAB_2919942
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP36776
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then processively degraded(PMID:15870080).

实验方案

Product Specific Protocols
IF protocol for CL594 LONP1 antibody CL594-66043Download protocol
Standard Protocols
Click here to view our Standard Protocols