验证数据展示
经过测试的应用
| Positive WB detected in | ROS1728 cells, HEK-293 cells, HSC-T6 cells, human heart tissue, human liver tissue, Jurkat cells, L02 cells, RAW 264.7 cells, NIH/3T3 cells |
| Positive IHC detected in | human lung cancer tissue, human heart tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | C6 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66043-1-Ig targets LONP1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag7306 Product name: Recombinant human LONP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 614-959 aa of BC000235 Sequence: EQNANFLDHYLDVPVDLSKVLFICTANVTDTIPEPLRDRMEMINVSGYVAQEKLAIAERYLVPQARALCGLDESKAKLSSDVLTLLIKQYCRESGVRNLQKQVEKVLRKSAYKIVSGEAESVEVTPENLQDFVGKPVFTVERMYDVTPPGVVMGLAWTAMGGSTLFVETSLRRPQDKDAKGDKDGSLEVTGQLGEVMKESARIAYTFARAFLMQHAPANDYLVTSHIHLHVPEGATPKDGPSAGCTIVTALLSLAMGRPVRQNLAMTGEVSLTGKILPVGGIKEKTIAAKRAGVTCIVLPAENKKDFYDLAAFITEGLEVHFVEHYREIFDIAFPDEQAEALAVER 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | lon peptidase 1, mitochondrial |
| 别名 | 1C6C12, EC:3.4.21.53, LON, LONP, LONP 1 |
| 计算分子量 | 106 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC000235 |
| 基因名称 | LONP1 |
| Gene ID (NCBI) | 9361 |
| RRID | AB_2881494 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P36776 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then processively degraded(PMID:15870080).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for LONP1 antibody 66043-1-Ig | Download protocol |
| IHC protocol for LONP1 antibody 66043-1-Ig | Download protocol |
| WB protocol for LONP1 antibody 66043-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B Miriplatin-loaded liposome, as a novel mitophagy inducer, suppresses pancreatic cancer proliferation through blocking POLG and TFAM-mediated mtDNA replication | ||
Dev Cell HMOX1-LDHB interaction promotes ferroptosis by inducing mitochondrial dysfunction in foamy macrophages during advanced atherosclerosis | ||
Mol Med ATF5 regulates tubulointerstitial injury in diabetic kidney disease via mitochondrial unfolded protein response | ||
J Cell Mol Med LONP1 alleviates ageing-related renal fibrosis by maintaining mitochondrial homeostasis | ||
J Physiol Biochem PGC-1α activation boosts exercise-dependent cellular response in the skeletal muscle |
