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验证数据展示

  • WB analysis using 66043-1-Ig (same clone as 66043-1-PBS)

    WB analysis using 66043-1-Ig (same clone as 66043-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66043-1-Ig (same clone as 66043-1-PBS)

    WB analysis of Jurkat using 66043-1-Ig (same clone as 66043-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66043-1-Ig (same clone as 66043-1-PBS)

    IHC staining of human lung cancer using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 66043-1-Ig (same clone as 66043-1-PBS)

    IHC staining of human lung cancer using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 66043-1-Ig (same clone as 66043-1-PBS)

    WB analysis of Jurkat using 66043-1-Ig (same clone as 66043-1-PBS)

    WB result of LONP1 antibody (66043-1-Ig; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-LONP1 transfected Jurkat cells. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • IF Staining of C6 using 66043-1-Ig (same clone as 66043-1-PBS)

    IF Staining of C6 using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunofluorescent analysis of C6 cells using 66043-1-Ig (LONP1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • IF Staining of C6 using 66043-1-Ig (same clone as 66043-1-PBS)

    IF Staining of C6 using 66043-1-Ig (same clone as 66043-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using LONP1 antibody (66043-1-Ig, Clone: 1C6C12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50672-1

    Cytometric bead array standard curve of MP50672-1

    Cytometric bead array standard curve of MP50672-1, LONP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66043-1-PBS. Detection antibody: 66043-2-PBS. Standard:Ag7306. Range: 1.563-100 ng/mL.

  • Featured Product
  • KD/KO验证

LONP1 Monoclonal antibody, PBS Only (Capture)

LONP1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66043-1-PBS

产品说明书

CloneNo. 1C6C12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

1C6C12, EC:3.4.21.53, LON, LONP, LONP 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66043-1-PBS targets LONP1 as part of a matched antibody pair:

MP50672-1: 66043-1-PBS capture and 66043-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7306

Product name: Recombinant human LONP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 614-959 aa of BC000235

Sequence: EQNANFLDHYLDVPVDLSKVLFICTANVTDTIPEPLRDRMEMINVSGYVAQEKLAIAERYLVPQARALCGLDESKAKLSSDVLTLLIKQYCRESGVRNLQKQVEKVLRKSAYKIVSGEAESVEVTPENLQDFVGKPVFTVERMYDVTPPGVVMGLAWTAMGGSTLFVETSLRRPQDKDAKGDKDGSLEVTGQLGEVMKESARIAYTFARAFLMQHAPANDYLVTSHIHLHVPEGATPKDGPSAGCTIVTALLSLAMGRPVRQNLAMTGEVSLTGKILPVGGIKEKTIAAKRAGVTCIVLPAENKKDFYDLAAFITEGLEVHFVEHYREIFDIAFPDEQAEALAVER

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 lon peptidase 1, mitochondrial
别名 1C6C12, EC:3.4.21.53, LON, LONP, LONP 1
计算分子量 106 kDa
观测分子量 100 kDa
GenBank蛋白编号BC000235
基因名称 LONP1
Gene ID (NCBI) 9361
RRIDAB_2881494
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP36776
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then processively degraded(PMID:15870080).

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