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验证数据展示

  • FC experiment of human PBMCs using CL647-98063

    FC experiment of human PBMCs using CL647-98063

    1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with PE Anti-Human CD8a and 5 ul CoraLite® Plus 647 Anti-Human LIGHT/CD258 Rabbit RecAb (CL647-98063, Clone: 240694D7). Cells were not fixed.

  • FC experiment of human PBMCs using CL647-98063

    FC experiment of human PBMCs using CL647-98063

    1x10^6 PMA, Ionomycin and Brefeldin A treated human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human LIGHT/CD258 Rabbit RecAb (CL647-98063, Clone: 240694D7) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human LIGHT/CD258 Rabbit RecAb (CL647-98063, Clone: 240694D7) (black, dashed). Cells were not fixed.

CoraLite® Plus 647 Anti-Human LIGHT/CD258 Rabbit Recombinant Antibody

LIGHT/CD258 Uni-rAbTM Recombinant Antibody for FC
Cat No. CL647-98063

产品说明书

CloneNo. 240694D7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

LIGHT, 240694D7, CD258, HVEM L, HVEML

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC detected inPMA and ionomycin treated human PBMCs
Planning an IHC experiment? We recommend our IHCeasy TNFSF14/LIGHT Ready-To-Use IHC Kit. TNFSF14/LIGHT primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 98063-1-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 5 ul per 10^6 cells in a 100 µl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-98063 targets LIGHT/CD258 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 LIGHT/CD258 fusion protein Eg32095 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor (ligand) superfamily, member 14
别名 LIGHT, 240694D7, CD258, HVEM L, HVEML
计算分子量26KD
GenBank蛋白编号NM_003807
基因名称 LIGHT
Gene ID (NCBI) 8740
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43557
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

LIGHT, also known as TNFSF14, HVEML, or CD258, is a type II transmembrane protein that belongs to the TNF superfamily (PMID: 9462508). It is predominantly expressed in lymphoid tissues and produced by activated T cells and immature dendritic cells (DCs) (PMID: 9462508; 10754304). Through its two primary functional receptors HVEM (TNFRSF14) and lymphotoxin β receptor (LTβR), LIGHT signaling is involved in development and maintenance of lymphoid tissues, as well as innate and adaptive immune responses (PMID: 24575096; 26720335). In addition to the membrane form, LIGHT can also exist as a soluble form generated by cleavage of the extracellular portion of the membrane form.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 LIGHT/CD258 antibody CL647-98063Download protocol
Standard Protocols
Click here to view our Standard Protocols