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验证数据展示

  • FC experiment of human PBMCs using 98063-1-RR (same clone as 98063-1-PBS)

    FC experiment of human PBMCs using 98063-1-RR (same clone as 98063-1-PBS)

    1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were stained with 0.25 ug Anti-Human LIGHT/CD258 Rabbit Recombinant Antibody (98063-1-RR, Clone:240694D7) and PE-Conjugated Goat Anti-Rabbit IgG (H+L). Cells were then stained with APC Anti-Human CD8a. Cells were not fixed. This data was developed using the same antibody clone with 98063-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98063-1-RR (same clone as 98063-1-PBS)

    FC experiment of human PBMCs using 98063-1-RR (same clone as 98063-1-PBS)

    1x10^6 untreated (black) or PMA, Ionomycin and Brefeldin A treated (red) human PBMCs were stained with 0.25 ug Anti-Human LIGHT/CD258 Rabbit Recombinant Antibody (98063-1-RR, Clone:240694D7) and PE-Conjugated Goat Anti-Rabbit IgG (H+L), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 98063-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98063-1-RR

    Affinity and Kinetic Characterization of 98063-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98063-1-RR against Human LIGHT/CD258 were performed. The affinity constant is 1.25 nM.

Anti-Human LIGHT/CD258 Rabbit Recombinant Antibody, PBS Only

LIGHT/CD258 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98063-1-PBS

产品说明书

CloneNo. 240694D7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

LIGHT, HVEM-L, HVEML, HVEM L, CD258

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TNFSF14/LIGHT Ready-To-Use IHC Kit. TNFSF14/LIGHT primary antibody included.

产品信息

98063-1-PBS targets LIGHT/CD258 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor (ligand) superfamily, member 14
别名 LIGHT, HVEM-L, HVEML, HVEM L, CD258
计算分子量26KD
GenBank蛋白编号NM_003807
基因名称 LIGHT
Gene ID (NCBI) 8740
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO43557
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LIGHT, also known as TNFSF14, HVEML, or CD258, is a type II transmembrane protein that belongs to the TNF superfamily (PMID: 9462508). It is predominantly expressed in lymphoid tissues and produced by activated T cells and immature dendritic cells (DCs) (PMID: 9462508; 10754304). Through its two primary functional receptors HVEM (TNFRSF14) and lymphotoxin β receptor (LTβR), LIGHT signaling is involved in development and maintenance of lymphoid tissues, as well as innate and adaptive immune responses (PMID: 24575096; 26720335). In addition to the membrane form, LIGHT can also exist as a soluble form generated by cleavage of the extracellular portion of the membrane form.