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验证数据展示

  • IF Staining of HepG2 using CL488-83073-2

    IF Staining of HepG2 using CL488-83073-2

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 LCMT2 antibody (CL488-83073-2, Clone: 230405H8 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-83073-2

    FC experiment of HeLa using CL488-83073-2

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Lcmt2 Recombinant Antibody (CL488-83073-2, Clone:230405H8)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite® Plus 488-conjugated LCMT2 Recombinant monoclonal antibody

LCMT2 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra)
Cat No. CL488-83073-2

产品说明书

CloneNo. 230405H8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

230405H8, EC:2.1.1.290, KIAA0547, PPM2, tRNA wybutosine-synthesizing protein 4

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 83073-2-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83073-2 targets LCMT2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag11145

Product name: Recombinant human LCMT2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 340-686 aa of BC015949

Sequence: VFPASVVSSEGQVPNLKRYGHASVFLSPDVILSAGGFGEQEGRHCRVSQFHLLSRDCDSEWKGSQIGSCGTGVQWDGRLYHTMTRLSESRVLVLGGRLSPVSPALGVLQLHFFKSEDNNTEDLKVTITKAGRKDDSTLCCWRHSTTEVSCQNQEYLFVYGGRSVVEPVLSDWHFLHVGTMAWVRIPVEGEVPEARHSHSACTWQGGALIAGGLGASEEPLNSVLFLRPISCGFLWESVDIQPPITPRYSHTAHVLNGKLLLVGGIWIHSSSFPGVTVINLTTGLSSEYQIDTTYVPWPLMLHNHTSILLPEEQQLLLLGGGGNCFSFGTYFNPHTVTLDLSSLSAGQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 leucine carboxyl methyltransferase 2
别名 230405H8, EC:2.1.1.290, KIAA0547, PPM2, tRNA wybutosine-synthesizing protein 4
计算分子量 aa, 76 kDa
GenBank蛋白编号BC015949
基因名称 LCMT2
Gene ID (NCBI) 9836
RRIDAB_3673179
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDO60294
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LCMT2, also known as tRNA wybutosine-synthesizing protein 4 (TYW4), is an S-adenosylmethionine (SAM)-dependent enzyme that catalyzes the final step of yW biosynthesis, methylation and methoxycarbonylation.LCMT2 has a conserved leucine carboxyl methyltransferase (LCM) domain in its N-terminal region (PMID: 16642040).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 LCMT2 antibody CL488-83073-2Download protocol
IF protocol for CL Plus 488 LCMT2 antibody CL488-83073-2Download protocol
Standard Protocols
Click here to view our Standard Protocols
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