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验证数据展示

  • IF Staining of HepG2 using 83073-2-RR

    IF Staining of HepG2 using 83073-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LCMT2 antibody (83073-2-RR, Clone: 230405H8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 83073-2-RR

    FC experiment of HeLa using 83073-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug LCMT2 Recombinant antibody (83073-2-RR, Clone:230405H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

LCMT2 Recombinant antibody

LCMT2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83073-2-RR

产品说明书

CloneNo. 230405H8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

tRNA yW-synthesizing protein 4, tRNA wybutosine-synthesizing protein 4, PPM2, KIAA0547, EC:2.1.1.290

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83073-2-RR targets LCMT2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 LCMT2 fusion protein Ag11145 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 leucine carboxyl methyltransferase 2
别名 tRNA yW-synthesizing protein 4, tRNA wybutosine-synthesizing protein 4, PPM2, KIAA0547, EC:2.1.1.290
计算分子量 aa, 76 kDa
GenBank蛋白编号BC015949
基因名称 LCMT2
Gene ID (NCBI) 9836
RRIDAB_3670792
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO60294
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LCMT2, also known as tRNA wybutosine-synthesizing protein 4 (TYW4), is an S-adenosylmethionine (SAM)-dependent enzyme that catalyzes the final step of yW biosynthesis, methylation and methoxycarbonylation.LCMT2 has a conserved leucine carboxyl methyltransferase (LCM) domain in its N-terminal region (PMID: 16642040).

实验方案

Product Specific Protocols
IF protocol for LCMT2 antibody 83073-2-RRDownload protocol
FC protocol for LCMT2 antibody 83073-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols