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验证数据展示

  • IF Staining of HepG2 using 83073-2-RR (same clone as 83073-2-PBS)

    IF Staining of HepG2 using 83073-2-RR (same clone as 83073-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LCMT2 antibody (83073-2-RR, Clone: 230405H8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83073-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83073-2-RR (same clone as 83073-2-PBS)

    FC experiment of HeLa using 83073-2-RR (same clone as 83073-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug LCMT2 Recombinant antibody (83073-2-RR, Clone:230405H8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83073-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00060-1

    Cytometric bead array standard curve of MP00060-1

    Cytometric bead array standard curve ofMP00060-1, LCMT2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83073-1-PBS. Detection antibody: 83073-2-PBS. Standard: Ag11145. Range: 0.625-20 ng/mL.

LCMT2 Recombinant antibody, PBS Only (Detector)

LCMT2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83073-2-PBS

产品说明书

CloneNo. 230405H8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

tRNA yW-synthesizing protein 4, tRNA wybutosine-synthesizing protein 4, PPM2, KIAA0547, EC:2.1.1.290

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83073-2-PBS targets LCMT2 as part of a matched antibody pair:

MP00060-1: 83073-1-PBS capture and 83073-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 LCMT2 fusion protein Ag11145 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 leucine carboxyl methyltransferase 2
别名 tRNA yW-synthesizing protein 4, tRNA wybutosine-synthesizing protein 4, PPM2, KIAA0547, EC:2.1.1.290
计算分子量 aa, 76 kDa
GenBank蛋白编号BC015949
基因名称 LCMT2
Gene ID (NCBI) 9836
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO60294
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C