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验证数据展示

  • IF Staining of mouse brain using CL594-67115

    IF Staining of mouse brain using CL594-67115

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 L1CAM antibody (CL594-67115, Clone: 3H7B9 ) at dilution of 1:200.

  • IF Staining of mouse brain using CL594-67115

    IF Staining of mouse brain using CL594-67115

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 L1CAM antibody (CL594-67115, Clone: 3H7B9 ) at dilution of 1:200.

CoraLite®594-conjugated L1CAM Monoclonal antibody

L1CAM Monoclonal Antibody for IF-P
Cat No. CL594-67115

产品说明书

CloneNo. 3H7B9

宿主/亚型

Mouse / IgG1

种属反应性

Human, Pig, Mouse, Rat

应用

IF-P

别名

CAML1, CD171, HSAS, HSAS1, L1 cell adhesion molecule, L1CAM, MASA, MIC5, N CAM L1, N CAML1, NCAM L1, S10, SPG1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy L1CAM Ready-To-Use IHC Kit. L1CAM primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67115-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67115 targets L1CAM in IF-P applications and shows reactivity with Human, Pig, Mouse, Rat samples.

经测试应用 IF-P Application Description
经测试反应性 Human, Pig, Mouse, Rat
免疫原 L1CAM fusion protein Ag17706 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 L1 cell adhesion molecule
别名 CAML1, CD171, HSAS, HSAS1, L1 cell adhesion molecule, L1CAM, MASA, MIC5, N CAM L1, N CAML1, NCAM L1, S10, SPG1
计算分子量 1257 aa, 140 kDa
观测分子量 220 kDa
GenBank蛋白编号BC126229
基因名称 L1CAM
Gene ID (NCBI) 3897
RRIDAB_2920087
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP32004
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).

实验方案

Product Specific Protocols
IF protocol for CL594 L1CAM antibody CL594-67115Download protocol
Standard Protocols
Click here to view our Standard Protocols