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验证数据展示

  • IF Staining of mouse brain using CL594-67115

    IF Staining of mouse brain using CL594-67115

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 L1CAM antibody (CL594-67115, Clone: 3H7B9 ) at dilution of 1:200.

  • IF Staining of mouse brain using CL594-67115

    IF Staining of mouse brain using CL594-67115

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 L1CAM antibody (CL594-67115, Clone: 3H7B9 ) at dilution of 1:200.

CoraLite®594-conjugated L1CAM/CD171 Monoclonal antibody

L1CAM/CD171 Monoclonal Antibody for IF-P
Cat No. CL594-67115

产品说明书

CloneNo. 3H7B9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF-P

别名

L1CAM, 3H7B9, CAML1, CD171, HSAS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy L1CAM Ready-To-Use IHC Kit. L1CAM primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67115-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67115 targets L1CAM/CD171 in IF-P applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat, pig
免疫原 L1CAM/CD171 fusion protein Ag17706 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 L1 cell adhesion molecule
别名 L1CAM, 3H7B9, CAML1, CD171, HSAS
计算分子量 1257 aa, 140 kDa
观测分子量 220 kDa
GenBank蛋白编号BC126229
基因名称 L1CAM
Gene ID (NCBI) 3897
RRIDAB_2920087
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP32004
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).

实验方案

Product Specific Protocols
IF protocol for CL594 L1CAM/CD171 antibody CL594-67115Download protocol
Standard Protocols
Click here to view our Standard Protocols