CoraLite® Plus 488-conjugated L1CAM/CD171 Monoclonal antibody

L1CAM/CD171 Monoclonal Antibody for IF-P
Cat No. CL488-67115

产品说明书

CloneNo. 3H7B9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF-P and More (1)

L1CAM, 3H7B9, CAML1, CD171, HSAS

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy L1CAM Ready-To-Use IHC Kit. L1CAM primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67115-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-67115 targets L1CAM/CD171 in IF-P applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF-P Application Description
文献引用应用IF
经测试反应性 human, mouse, rat, pig
文献引用反应性human
免疫原 L1CAM/CD171 fusion protein Ag17706 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 L1 cell adhesion molecule
别名 L1CAM, 3H7B9, CAML1, CD171, HSAS
计算分子量 1257 aa, 140 kDa
观测分子量 220 kDa
GenBank蛋白编号BC126229
基因名称 L1CAM
Gene ID (NCBI) 3897
RRIDAB_2919423
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP32004
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 L1CAM/CD171 antibody CL488-67115Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Extracell Vesicles

Isolation and quantification of L1CAM-positive extracellular vesicles on a chip as a potential biomarker for Parkinson's Disease

Authors - Danyu Li
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