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CoraLite®594-conjugated KU70,XRCC6 Monoclonal antibody

KU70,XRCC6 Monoclonal Antibody for FC (Intra), IF/ICC, WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

FC (Intra), IF/ICC, WB

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3C12D9

Cat No : CL594-66607

Print datasheet

Synonyms

5 dRP lyase Ku70, 70 kDa subunit of Ku antigen, CTC75, CTCBF, DNA repair protein XRCC6, G22P1, KU70, KU70,XRCC6, ML8, Thyroid lupus autoantigen, TLAA, XRCC6

验证数据展示

  • WB analysis using CL594-66607

    WB analysis using CL594-66607

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-66607 (KU70,XRCC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using CL594-66607

    IF Staining of HeLa using CL594-66607

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 KU70,XRCC6 antibody (CL594-66607, Clone: 3C12D9 ) at dilution of 1:600.

  • FC experiment of HeLa using CL594-66607

    FC experiment of HeLa using CL594-66607

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human KU70,XRCC6 (CL594-66607, Clone:3C12D9) (red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells
Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy XRCC6 Ready-To-Use IHC Kit. XRCC6 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66607-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:300-1:1200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66607 targets KU70,XRCC6 in WB, IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC, WB
Tested Reactivity Human, mouse, rat
Immunogen KU70,XRCC6 fusion protein Ag21301 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name X-ray repair complementing defective repair in Chinese hamster cells 6
Synonyms 5 dRP lyase Ku70, 70 kDa subunit of Ku antigen, CTC75, CTCBF, DNA repair protein XRCC6, G22P1, KU70, KU70,XRCC6, ML8, Thyroid lupus autoantigen, TLAA, XRCC6
Calculated Molecular Weight 75 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC008343
Gene Symbol XRCC6
Gene ID (NCBI) 2547
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP12956
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

X-ray repair cross-complementing protein 6(XRCC6)gene encodes subunit p70 of the p70/p80 autoantigen which has a role in DNA repair or transposition. DNA helicase II complex binds preferentially to fork-like ends of double-standed DNA in a cell cycle-dependent manner, which may be mediated by XRCC6. XRCC6/5 can form a heterdimer, which function as a regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA, and also it can stablize broken DNA ends and bringing them together. Otherwise, XRCC5/6 heterdimer together with APEX1 can negatively regulate of transcription.

实验方案

Product Specific Protocols
WB protocol for CL594 KU70,XRCC6 antibody CL594-66607Download protocol
IF protocol for CL594 KU70,XRCC6 antibody CL594-66607Download protocol
FC protocol for CL594 KU70,XRCC6 antibody CL594-66607Download protocol
Standard Protocols
Click here to view our Standard Protocols