KU70,XRCC6 Monoclonal antibody
KU70,XRCC6 Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (1)
Applications
FC, IF/ICC, IHC, WB, ELISA and More (3)
Conjugate
Unconjugated
CloneNo.
3C12D9
验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, Neuro-2a cells |
Positive IHC detected in | human breast cancer tissue, human colon cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, human breast cancer tissue, HepG2 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Immunofluorescence (IF) | IF : 1:500-1:2000 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66607-1-Ig targets KU70,XRCC6 in WB, IP, IF, FC, IHC, CoIP, ChIP, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | FC, IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IP, IF, CoIP, ChIP |
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | KU70,XRCC6 fusion protein Ag21301 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | X-ray repair complementing defective repair in Chinese hamster cells 6 |
Synonyms | 5 dRP lyase Ku70, 70 kDa subunit of Ku antigen, CTC75, CTCBF, DNA repair protein XRCC6, G22P1, KU70, KU70,XRCC6, ML8, Thyroid lupus autoantigen, TLAA, XRCC6 |
Calculated Molecular Weight | 75 kDa |
Observed Molecular Weight | 70 kDa |
GenBank Accession Number | BC008343 |
Gene Symbol | XRCC6 |
Gene ID (NCBI) | 2547 |
RRID | AB_2881967 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P12956 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for KU70,XRCC6 antibody 66607-1-Ig | Download protocol |
IHC protocol for KU70,XRCC6 antibody 66607-1-Ig | Download protocol |
IF protocol for KU70,XRCC6 antibody 66607-1-Ig | Download protocol |
FC protocol for KU70,XRCC6 antibody 66607-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Immunity Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation. | ||
Nucleic Acids Res Interactome of intact chromatosome variants with site-specifically ubiquitylated and acetylated linker histone H1.2 | ||
Cancer Lett ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer | ||
J Nanobiotechnology Minimizing adverse effects of Cerenkov radiation induced photodynamic therapy with transformable photosensitizer-loaded nanovesicles. | ||
Cell Rep circPRKAA1 activates a Ku80/Ku70/SREBP-1 axis driving de novo fatty acid synthesis in cancer cells |